Study M547
Study name | Li JG 2019 |
Title | Altered gut metabolome contributes to depression-like behaviors in rats exposed to chronic unpredictable mild stress |
Overall design | In this study, the authors performed a temporal dynamic gas chromatography-mass spectrometry (GC-MS) analysis of the fecal and plasma metabolomes during the development of depressive-like behaviors in chronic unpredictable mild stress (CUMS) rats. UHPLC-ESI-MS/MS quantitation of 12 neurotransmitters in the hippocampus was carried out. Sprague-Dawley rats were divided into the following 2 groups (n = 6 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 12 |
Tissue | Peripheral; Faece; Faece; Peripheral; Blood; Plasma; Central; Brain; Hippocampus; Central; Brain; Prefrontal cortex; |
Platform | MS-based; LC-MS: Ultra-high performance liquid chromatography-Electrospray Ionization -Tandem Mass Spectrometry (UHPLC-ESI-MS/MS); MS-based; GC-MS: trace gas chromatograph coupled with a Polyris Q Ion Trap mass spectrometer (Thermo Fisher Scientific, MA, USA); |
PMID | |
DOI | |
Citation | Li J, Jia X, Wang C, et al. Altered gut metabolome contributes to depression-like behaviors in rats exposed to chronic unpredictable mild stress. Transl Psychiatry. 2019;9(1):40. |
Metabolite | myo-Inositol; Glycerol; L-Valine; Glycine; L-Serine; L-Threonine; L-Isoleucine; L-Lactic acid; L-Alanine; L-Aspartic acid; L-Tyrosine; D-Arabinose; Phosphoric acid; L-Malic acid; 2-Hydroxyglutarate; Sarcosine; Methylamine; Benzoic acid; Butyric acid; Propionic acid; Alpha-Linolenic acid; Lactose; Allose; Isoflurane; D-Rhamnose; Terephthalic acid; |
