Study M514
Study name | Weckmann K 2019 |
Title | Ketamine’s effects on the glutamatergic and GABAergic systems: a proteomics and metabolomics study in mice |
Overall design | The authors carried out a time-dependent targeted mass spectrometry-based metabolomics profiling analysis combined with a quantitative based on in vivo 15N metabolic labeling proteome comparison of ketamine- and vehicle-treated mice. The metabolomics and proteomics datasets were used to further elucidate ketamine’s mode of action on the gamma-aminobutyric acid (GABA)ergic and glutamatergic systems. The floating time was scored 2 h, 14 h, 24 h, and 72 h after ketamine treatment. Hippocampal glutamatergic and GABAergic system-related metabolites were measured after a single injection of a low dose of ketamine (3 mg/kg, n = 5 per group and time point). |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | 40 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Prominence UFLC high-performance LC system (Shimadzu, Columbia, SC, USA) with 5500 QTRAP triple quadrupole mass spectrometer (AB/SCIEX, Framingham, MA, USA); |
PMID | |
DOI | |
Citation | Weckmann K, Deery MJ, Howard JA, et al. Ketamine’s effects on the glutamatergic and GABAergic systems: a proteomics and metabolomics study in mice. Mol Neuropsychiatry. 2019 Mar;5(1):42-51. |
Metabolite | Gamma-Aminobutyric acid; L-Glutamic acid; |
