Study M498
Study name | Smaga I 2014 |
Title | Antidepressants and changes in concentration of endocannabinoids and N-acylethanolamines in rat brain structures |
Overall design | The aim of this study was to investigate the effect of acutely or chronically administering antidepressants [imipramine (IMI) (15 mg/kg), escitalopram (ESC) (10 mg/kg), and tianeptine (10 mg/kg)] on the levels of both eCBs [anandamide (AEA) and 2-arachidonoylglycerol (2-AG)] and N-acylethanolamines (NAEs) [palmitoylethanolamide (PEA) and oleoylethanolamide (OEA)] in various rat brain regions (n = 8 each group). We also examined the ability of the acute and chronic administration of N-acetylcysteine (NAC) (a mucolytic drug; 100 mg/kg) or URB597 (a fatty acid amide hydrolase inhibitor; 0.3 mg/kg), which have both elicited antidepressant activity in preclinical studies, to affect eCB and NAE levels. Next, the authors determined whether the observed effects are stable 10 days after the chronic administration of these drugs was halted. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 120 |
Tissue | Central; Brain; Dorsal striatum; Central; Brain; Prefrontal cortex; Central; Brain; Frontal cortex; Central; Brain; Hippocampus; Central; Brain; Nucleus accumbens; Central; Brain; Cerebellum; |
Platform | MS-based; LC-MS: Agilent 1100 (Agilent Technologies, Waldbronn, Germany) LC system with Applied Biosystems MDS Sciex (Concord, ON, Canada) API 2000 triple quadruple mass spectrometer; |
PMID | |
DOI | |
Citation | Smaga I, Bystrowska B, Gawlinski D, et al. Antidepressants and changes in concentration of endocannabinoids and N-acylethanolamines in rat brain structures. Neurotox Res. 2014;26(2):190-206. |
Metabolite |
