Study M481
Study name | Mishra PK 2018 |
Title | Subanesthetic ketamine reverses neuronal and astroglial metabolic activity deficits in a social defeat model of depression |
Overall design | This study explores the impact of ketamine on neuronal and astroglial metabolic activity in prefrontal cortex in a social defeat (SD) model of depression. C57BL/6 mice were subjected to a social defeat paradigm for 5 min a day for 10 consecutive days. Ketamine (10 mg/kg, intraperitoneal) was administered to mice for two consecutive days following the last defeat stress. Mice were infused with [1,6-13 C2 ]glucose or [2-13 C]acetate to assess neuronal and astroglial metabolic activity, respectively, together with proton-observed carbon-edited nuclear magnetic resonance spectroscopy in prefrontal cortex tissue extract. Mice were divided into the following 4 groups: (1) control group (n = 11), (2) ketamine group (ketamine treatment at the dose of 10 mg/kg on day 12 and 13 intraperitoneally. n = 13), (3) SD group (social defeat model, n = 12), (4) SD + ketamine group (social defeat model plus ketamine treatment at the dose of 10 mg/kg on day 12 and 13 intraperitoneally, n = 13). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Social defeat model; Social defeat model; |
Criteria for depression | Sucrose preference test, social interaction test, forced swimming test |
Sample size | 49 |
Tissue | Peripheral; Blood; Plasma; Central; Brain; Prefrontal cortex; |
Platform | NMR; NMR: 600 MHz NMR spectrometer (AVANCE II, Bruker Biospin, Karlsruhe, Germany); |
PMID | |
DOI | |
Citation | Mishra PK, Kumar A, Behar KL, et al. Subanesthetic ketamine reverses neuronal and astroglial metabolic activity deficits in a social defeat model of depression. J Neurochem. 2018;146(6):722-34. |
Metabolite |
