Study M478
Study name | Lian B 2018 |
Title | Mechanisms of ketamine on mice hippocampi shown by gas chromatography-mass spectrometry-based metabolomic analysis |
Overall design | In the present study, we used a gas chromatography-mass spectrometry-based metabolomics method to evaluate the effects of ketamine on mice hippocampi. Multivariate statistical analysis and ingenuity pathway analysis were then used to identify and explore the potential mechanisms and biofunction of ketamine. Mice were assigned randomly to each of the following four treatment groups (n = 10 each): (1) control group: treated with saline at a single dose of 1 ml/kg, intraperitoneally; (2) ketamine group: treated with ketamine at a single dose of 10 mg/kg, intraperitoneally; (3) NBQX group: treated with 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) at a single dose of 10 mg/kg, intraperitoneally; and (4) ketamine + NBQX group: pretreated with NBQX 30 min before the treatment of ketamine, both at a single dose of 10 mg/kg, intraperitoneally. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | 40 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; GC-MS: Agilent 7980 GC system (Agilent Technologies Inc., USA); |
PMID | |
DOI | |
Citation | Lian B, Xia J, Yang X, et al. Mechanisms of ketamine on mice hippocampi shown by gas chromatography-mass spectrometry-based metabolomic analysis. Neuroreport. 2018;29(9):704-11. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; Glycine; L-Alanine; L-Glutamine; |
