Study M450
Study name | Wang JJ 2018 |
Title | Regulation of the kynurenine metabolism pathway by Xiaoyao San and the underlying effect in the hippocampus of the depressed rat |
Overall design | The present study aimed to investigate the antidepressant effects of Xiaoyao San (XYS) in a rat model of chronic unpredictable mild stress (CUMS) and the underlying mechanisms. Rats were divided into the following 4 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + high dose of XYS group (stressor plus XYS treatment at the dose of 10.53 g/kg), and (4) CUMS + paroxetine group (stressor plus paroxetine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via oral administration daily for 14 days after the model building period. The concentrations in rat plasma of tryptophan (Trp) and its metabolic products, including kynurenine (Kyn) and quinolinic acid (QUIN), were determined using high performance liquid chromatography tandem mass spectrometry with electrochemical detection (HPLC-MS/MS). Six samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 24 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Agilent 1200 HPLC system with Agilent 6410 triple quadrupole mass spectrometer (Agilent Technologies, Inc., CA, USA); |
PMID | |
DOI | |
Citation | Wang J, Li X, He S, et al. Regulation of the kynurenine metabolism pathway by Xiaoyao San and the underlying effect in the hippocampus of the depressed rat. J Ethnopharmacol 2018;214:13-21. |
Metabolite |
