Study M177
Study name | Chowdhury GM 2017 |
Title | Transiently increased glutamate cycling in rat PFC is associated with rapid onset of antidepressant-like effects |
Overall design | The aim of this study was to demonstrate that the antidepressant-like efficacy of three unique drugs, with reported rapid onset antidepressant properties, is coupled with a rapid transient rise in glutamate cycling in the medial prefronal cortex (mPFC) of awake rats as measured by ex vivo 1H-[13C]-nuclear magnetic resonance spectroscopy. Rats were acutely pretreated by intraperitoneal injection with a single dose of ketamine (1, 3, 10, 30 and 80 mg/kg), Ro 25-6981 (1, 3 and 10 mg/kg), scopolamine (5, 25 and 100 ug/kg) or vehicle (controls) (n = 6-8 per group). At fixed times after drug injection, animals received an intravenous infusion of [1,6-13C2]glucose for 8 min to enrich the amino-acid pools of the brain with 13C, followed by rapid euthanasia. The mPFC was dissected, extracted with ethanol and metabolite 13C enrichments were measured. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | N/A |
Tissue | Central; Brain; Medial prefrontal cortex; Peripheral; Blood; Plasma; |
Platform | NMR; NMR: Bruker AVANCE spectrometer (Bruker Instruments, Billerica, MA); |
PMID | |
DOI | |
Citation | Chowdhury GM, Zhang J, Thomas M, et al. Transiently increased glutamate cycling in rat PFC is associated with rapid onset of antidepressant-like effects. Mol Psychiatry 2017;22(1):120-126. |
Metabolite |
