Study M171
Study name | Zhu XX 2015 |
Title | Danzhi Xiaoyao San ameliorates depressive-like behavior by shifting toward serotonin via the downregulation of hippocampal indoleamine 2,3-dioxygenase |
Overall design | The aim of this study was to investigate the hypothesis that Danzhi Xiaoyao San (DXS) treats depressive-like behavior by shifting the balance of the kynurenine (Kyn)/serotonin (5-HT) pathway toward the 5-HT pathway through the downregulation of hippocampal indoleamine 2,3-dioxygenase (IDO). Rats were divided into the following 4 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + DXS group (stressor plus Danzhi Xiaoyao San treatment at the dose of 4 g/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 2 mg/kg). Cytokines, IDO, and tryptophan (Trp) catabolites were analyzed by enzyme-linked immunosorbent assay, western blot, and liquid chromatography-electrospray ionization tandem mass spectrometry, respectively. The CUMS stress procedure lasted for 3 weeks, and drugs were administered by gavage using a tube twice a day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 40 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Prominence 20A series UFLC System (Shimadzu, Kyoto, Japan) with API4000 Qtrap MS system (ABSciex, Massachusetts, USA); |
PMID | |
DOI | |
Citation | Zhu X, Jing L, Chen C, et al. Danzhi Xiaoyao San ameliorates depressive-like behavior by shifting toward serotonin via the downregulation of hippocampal indoleamine 2,3-dioxygenase. J Ethnopharmacol 2015;160:86-93. |
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