Study M168
Study name | Ruda-Kucerova J 2015 |
Title | Reward related neurotransmitter changes in a model of depression: an in vivo microdialysis study |
Overall design | The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. Rats were divided into the following 4 groups (1) control group (sham-operated rats treated with saline as a vehicle; n = 5), (2) METH group (sham-operated rats treated with methamphetamine at a single dose of 5 mg/kg; n = 8), (3) OBX group (olfactory bulbectomized rats treated with saline; n = 6), (4) OBX + METH group (olfactory bulbectomized rats treated with methamphetamine at a single dose of 5 mg/kg; n = 8). Microdialysis experiments were carried out 3 weeks after the surgery. Methamphetamine was administered intraperitoneally (at 60 min) and dialysates were collected every 20 min. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Behavioural assay |
Sample size | 27 |
Tissue | Central; Cerebrospinal fluid; Cerebrospinal fluid; |
Platform | MS-based; LC-MS: chromatograph Accela 1250 autosampler with TSQ Vantage mass spectrometer (Thermo Scientific, USA); |
PMID | |
DOI | |
Citation | Ruda-Kucerova J, Amchova P, Havlickova T, et al. Reward related neurotransmitter changes in a model of depression: an in vivo microdialysis study. World J Biol Psychiatry 2015;16(7):521-35. |
Metabolite | Gamma-Aminobutyric acid; L-Glutamic acid; Homovanillic acid; 5-Hydroxyindoleacetic acid; 3,4-Dihydroxybenzeneacetic acid; |
