Study M165
Study name | Yang P 2014a |
Title | Alterations of amino acid level in depressed rat brain |
Overall design | The aim of this study was to discover new biomarkers for laboratory diagnostics of depression through determining and analyzing the levels of amino acids in rats' brain as well as providing potentially appropriate amino acids in plasma for future clinical diagnosis of depression and improvement of antidepressants. The determination of 34 amino acids through entire rats' brain was conducted in this study in order to demonstrate more possibilities for biomarkers of depression by discovering other potential amino acids in more areas of rats' brain using LC/MS platform. Rats were divided into the following 3 groups (n = 7 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 0.3 mg). The CUMS stress procedure lasted for 32 days, and fluoxetine was fed for another 30 days. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 21 |
Tissue | Central; Brain; Brain; |
Platform | MS-based; LC-MS: Ultimate3000 HPLC (DIONEX, USA) and QTRAP LC-MS/MS 3200 (AB SCIEX, USA) with Agilent 6460 Triple Quad LC/MS; |
PMID | |
DOI | |
Citation | Yang P, Li X, Ni J, et al. Alterations of amino acid level in depressed rat brain. Korean J Physiol Pharmacol 2014; 18(5): 371-6. |
Metabolite |
