Study M151
Study name | Eskelund A 2016 |
Title | Female Flinders Sensitive Line rats show estrous cycle-independent depression-like behavior and altered tryptophan metabolism |
Overall design | The aim of this study was to investigate 13 kynurenine and serotonin metabolites in female Flinders Sensitive Line (FSL) rats, a genetic rat model of depression. FSL rats and controls (Flinders Resistant Line rats), 12-20 weeks old, were subject to the forced swim test (FST), a commonly used measure of depression-like behavior. Open field was used to evaluate locomotor ability and agoraphobia. Tryptophan, kynurenine, 3-hydroxykynurenin, 5-hydroxytryptophan, 5-HT, nicotinamide, anthranilate, quinolinic acid and kynurenate were measured and analyzed for both brain and plasma using LC-MS. In Experiment 1, the female rats were divided into the following 2 groups: (1) Female FSL group (n = 31), (2) Female FRL group (n = 28). Brain and plasma tissues were collected for LC-MS analysis. In Experiment 2, the male rats were divided into the following 2 groups: (1) Male FSL group (n = 6), (2) Male FRL group (n = 6). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 71 |
Tissue | Central; Brain; Brain; Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Eskelund A, Budac DP, Sanchez C, et al. Female Flinders Sensitive Line rats show estrous cycle-independent depression-like behavior and altered tryptophan metabolism. Neuroscience 2016; 329:337-48. |
Metabolite | Hydroxykynurenine; |
