Study M146

Study name

Zhao SJ 2017

Title

[Regulation of seabuckthorn seed oil on metabolic pathway disturbances on rats exposed to chronic unpredictable mild stress]

Overall design

The aim of this study was to analyze and explore the changes of the metabolites in serum and urine of the chronic unpredictable depression rat model (CUMS) rats disturbed by seabuckthorn seed oil (SSO) with using nuclear magnetic resonance spectroscopy (1H NMR) and KEGG database. CUMS was prepared and the rats seabuckthorn seed oil was ig given to intervene at the same time for four weeks. Then the serum and the urine of rats were collected to test by 1HNMR based metabolomics approach which purpose was to analyze the changes of the metabolites. All the rats were divided into the following 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 50 mg/kg), (4) CUMS + low dose of SSO group (stressor plus seabuckthorn seed oil treatment at the dose of 0.35 g/kg), (5) CUMS + middle dose of SSO group (stressor plus seabuckthorn seed oil treatment at the dose of 0.70 g/kg), and (6) CUMS + high dose of SSO group (stressor plus seabuckthorn seed oil treatment at the dose of 1.40 g/kg). Six samples per group were used for metabonomic analysis. The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

36

Tissue

Peripheral; Urine; Urine;

Peripheral; Blood; Serum;

Platform

NMR; NMR: Bruker 600-MHz AVANCE III NMR;

PMID

N/A

DOI

10.7501/j.issn.0253-2670.2017.13.015

Citation

Zhao S, Zhao X, Xiang H, et al. [Regulation of seabuckthorn seed oil on metabolic pathway disturbances on rats exposed to chronic unpredictable mild stress]. Chinese Traditional and Herbal Drugs 2017; 48(13):2682-90. (Article in Chinese)

Metabolite

Citric acid;

3-Hydroxybutyric acid;

L-Phenylalanine;

L-Lactic acid;

L-Alanine;

L-Aspartic acid;

Succinic acid;

Oxoglutaric acid;

N-acetyl glycoprotein;

Betaine;

Beta-D-Glucose;

Trimethylamine N-oxide;

Acetic acid;

alpha-D-Glucose;

Lipid;

Trimethylamine;