Study M137
Study name | Zhai XJ 2015c |
Title | LC-MS/MS based studies on the anti-depressant effect of hypericin in the chronic unpredictable mild stress rat model |
Overall design | The aim of this study was to investigate the anti-depression effects of hypericin on chronic unpredictable mild stress (CUMS) model rats and identify the possible mechanisms. In this study, the protective effects of HY on CUMS-induced depression in rats were investigated by using a combination of behavioral assessments and urinary metabolites analysis. Urinary metabolites analyses were performed using LC-MS/MS. The plasma corticosterone concentration was also measured using LC-MS/MS. During the procedure of experiment, food consumption, body weight, adrenal gland, thymus and spleen indices, behavior scores, sucrose consumption, and stress hormone levels were measured. All the rats were divided into 4 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + hypericin group (stress plus hypericin treatment at the dose of 1.56 mg/kg), and (4) CUMS + venlafaxine group (stress plus venlafaxine treatment at the dose of 7.81 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered once a day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 24 |
Tissue | Peripheral; Urine; Urine; Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Shimadzu LC-30AD pump (Shimadzu, Kyoto, Japan) with API QTRAP 5500 triple quadrupole mass spectrometer (AB/MDSSciex, Ontario, Canada); |
PMID | |
DOI | |
Citation | Zhai XJ, Chen F, Chen C, et al. LC-MS/MS based studies on the anti-depressant effect of hypericin in the chronic unpredictable mild stress rat model. J Ethnopharmacol 2015; 169:363-9. |
Metabolite | L-Tryptophan; L-Valine; L-Proline; L-Glutamic acid; |
