Study M117

Study name

Yu M 2017

Title

Variations in gut microbiota and fecal Metabolic phenotype associated with depression by 16S rRNA gene sequencing and LC/MS-based Metabolomics

Overall design

To assess the impact of gut microbiota on fecal metabolic phenotype in depressive conditions, an integrated approach of 16S rRNA gene sequencing combined with ultra high-performance liquid chromatography-mass spectrometry (UHPLC-MS) based metabolomics was performed in chronic variable stress (CVS)-induced depression rat model. Rats were randomly divided into 2 groups (n = 8 in each group): (1) control group, (2) CVS group. The CVS stress procedure lasted for 4 weeks.

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Wistar rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

16

Tissue

Peripheral; Faece; Faece;

Platform

MS-based; LC-MS: Waters Acquity Ultra high Performance LC system (Waters Corp., Milford, USA) with Waters SYNAPT G2 HDMS (Waters Corp., Manchester, UK) TOF mass spectrometer;

PMID

28219800

DOI

10.1016/j.jpba.2017.02.008

Citation

Yu M, Jia H, Zhou C, et al. Variations in gut microbiota and fecal metabolic phenotype associated with depression by 16S rRNA gene sequencing and LC/MS-based metabolomics. J Pharm Biomed Anal 2017;138:231-9.

Metabolite

Cholic acid;

Chenodeoxycholic acid;

Hypoxanthine;

Nicotinic acid;

5-Methoxytryptophan;

3-Methyldioxyindole;

Deoxycholic acid;

Dextrorphan O-glucuronide;

Urobilin;

PE(14:1(9Z)/14:1(9Z));

PS(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z));

Muricholic acid;

L-Urobilinogen;

MG(0:0/20:3(5Z,8Z,11Z)/0:0);

MG(0:0/20:4(8Z,11Z,14Z,17Z)/0:0);

Stearyl citrate;