Study M1140
Study name | Tan JX 2023 |
Title | Multiomics approach to explore the different amelioration mechanisms of Chaihu Shugan San and Hypericum perforatum L. on the metabolic disorder of CUMS-induced depressive rats |
Overall design | The aim of this study was to explore potential antidepressant mechanisms of Chaihu Shugan San and Hypericum perforatum L. by analyzing ultra-performance liquid chromatography mass spectrometry metabolomics in chronic unpredictable mild stress (CUMS) rats. Sprague-Dawley rats were divided into the following 4 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + Chaihu Shugan San group (stress with Chaihu Shugan San treatment at the dose of 19.8 g/kg), and (4) CUMS + Hypericum perforatum L. group (stress with Hypericum perforatum L. treatment at the dose of 204 mg/kg). The CUMS stress procedure lasted for 12 weeks, and drugs were administered via intragastric once per day during the last 8 weeks of the model building period. Serum samples were used for global metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 24 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: ExionLC AD with QTRAP 6500+ mass spectrometer; |
PMID | |
DOI | |
Citation | Tan J, Li L, Li X, et al. Multiomics approach to explore the different amelioration mechanisms of Chaihu Shugan San and Hypericum perforatum L. on the metabolic disorder of CUMS-induced depressive rats. Phytomedicine Plus 2023;100409. |
Metabolite | 3-Hydroxybutyric acid; Uridine; Phenylacetylglycine; Trimethylamine N-oxide; L-Histidine; Deoxyadenosine; Malonic acid; Carnitine C16:1; Carnitine C20:2; Carnitine C18:1-OH; Glycoursodeoxycholic acid; Hexanoylcarnitine; 2-Octenoylcarnitine; Eicosapentaenoic acid; 15-HETE; m-Coumaric acid; 2-Methylhippuric acid; 12,13-EpOME; 9-OxoODE; 13-OxoODE; 9,10-Epoxystearic acid; 4-Methoxycinnamic acid; Carnitine C18:1; 5'-Deoxyadenosine; N-Methyl-D-aspartic acid; (+/-)12-HETE; 13-HOTE; 7-Ketodeoxycholic acid; Carnitine C13:0; Carnitine C13:1; 3'-Sialyllactose; (+/-)16-HETE; 14S-Hdha; 9,10-EpOME; 13(R)-HODE; Glycine deoxycholic acid; PysoPE 18:3; Glycohyodeoxycholic acid; 1-Amino-propan-2-ol; Carnitine C7:0; Docosaenoic acid; 3-(Pyrazol-1-yl)-L-alanine; Hydroxyoctadecadienylcarnitine; Carnitine C14:1-OH; |
