Study M1131

Study name

Walton NL 2023

Title

Impaired endogenous neurosteroid signaling contributes to behavioral deficits associated with chronic stress

Overall design

The aim of this study was to explore whether there are deficits in endogenous neurosteroid signaling following chronic unpredictable stress (CUS). C57BL/6J mice were divided into the following 2 groups: (1) control group, and (2) CUS group. The CUS stress procedure lasted for 3 weeks. Allopregnanolone levels in plasma, whole-brain, and basolateral amygdala samples from control and CUS mice were measured after stress using liquid chromatography with tandem mass spectrometry (n =4-13/group).

Study Type

Type1;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Forced swimming test, tail suspension test

Sample size

24

Tissue

Peripheral; Blood; Plasma;

Central; Brain; Brain;

Central; Brain; Basolateral amygdala;

Platform

MS-based; LC-MS: Sciex-Eksigent LC 200 with Sciex 5500 Qtrap mass spectrometer;

PMID

36736870

DOI

10.1016/j.biopsych.2023.01.022

Citation

Walton NL, Antonoudiou P, Barros L, et al. Impaired endogenous neurosteroid signaling contributes to behavioral deficits associated with chronic stress. Biol Psychiatry. 2023 Aug 1;94(3):249-261.

Metabolite

Allopregnanolone;