Study M112
Study name | Park DI 2017 |
Title | Delineation of molecular pathway activities of the chronic antidepressant treatment response suggests important roles for glutamatergic and ubiquitin-proteasome systems |
Overall design | The aim of this study was to identify molecular pathways related to antidepressant response. Paroxetine was administered to the DBA/2J mice for 28 days. Following the treatment, the mice were grouped into responders or non-responders depending on the time they spent immobile in the forced swim test. Paroxetine-treated long-time floating (PLF) and paroxetine-treated short-time floating (PSF) groups were stratified as proxies for drug non-responder and responder mice, respectively. Hippocampus and prefrontal cortex metabolites were extracted and analyzed with targeted metabolomics. Mice were divided into 2 groups: (1) control group, (2) paroxetine group (paroxetine treatment at the dose of 5 mg/kg for 4 weeks twice a day via customized palatable pellets). Paroxetine group were divided into responder (short-time floating, PSF) and non-responder (long-time floating, PLF) mice according to their forced swim test floating time. Five samples per group were used for metabonomic analysis. |
Study Type | Type5; |
Data available | https://www.nature.com/articles/tp201739#supplementary-information |
Organism | Mouse; DBA/2J mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | 10 |
Tissue | Central; Brain; Hippocampus; Central; Brain; Prefrontal cortex; |
Platform | MS-based; LC-MS: Prominence UFLC high-performance liquid chromatography system (Shimadzu, Columbia, MD, USA) with 5500 QTRAP triple quadrupole mass spectrometer (AB/SCIEX, Framingham, MA, USA); |
PMID | |
DOI | |
Citation | Park DI, Dournes C, Sillaber I, et al. Delineation of molecular pathway activities of the chronic antidepressant treatment response suggests important roles for glutamatergic and ubiquitin-proteasome systems. Transl Psychiatry 2017;7(4):e1078. |
Metabolite |
