Study M1086

Study name	Xie J 2023a
Title	Multi-omics data reveals the important role of glycerophospholipid metabolism in the crosstalk between gut and brain in depression
Overall design	The aim of this study was to observe the characteristics of gut microbiota, lipid metabolism and neurotransmitters in gut-liver-brain axis in the chronic unpredictable mild stress (CUMS) mice model of depression. C57BL/6 mice were divided into the following 2 groups (n = 10 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. The lipidomics analyses of fecal and liver samples were performed using liquid chromatography-mass spectrometry. The neurotransmitters in GABAergic and Catecholaminergic pathways were detected in the hippocampus of mice.
Study Type	Type1;
Data available	Unavailable
Organism	Mouse; C57BL/6 mouse;
Categories of depression	Animal model; Chronic mild stress model; Chronic mild stress model;
Criteria for depression	Sucrose preference test, forced swimming test
Sample size	20
Tissue	Central; Brain; Hippocampus; Peripheral; Faece; Faece; Peripheral; Liver; Liver;
Platform	MS-based; LC-MS: ACQUITY I Class UPLC system with Waters G2-S QTOF system (Waters, Milford, MA, USA);
PMID	36750892
DOI	10.1186/s12967-023-03942-w
Citation	Xie J, Zhong Q, Wu WT, et al. Multi-omics data reveals the important role of glycerophospholipid metabolism in the crosstalk between gut and brain in depression. J Transl Med. 2023 Feb 7;21(1):93.
Metabolite	Gamma-Aminobutyric acid; L-Phenylalanine; L-Glutamic acid; L-Glutamine; LysoPC(16:0); Vanillylmandelic acid; Acetic acid; Norepinephrine; Phenylethylamine; LysoPC(18:0); LysoPC(18:1); LysoPE(18:1); PE(36:4); LysoPE(16:0); LysoPE(18:0); PC(32:0); PC(36:2); Deoxycholic acid; Butyric acid; PC(16:0/18:2); Propionic acid; LysoPE(20:0); Cer(d18:1/18:0); PI(38:6); PS(38:4); PI(36:2); DG(34:2); LysoPI(18:0); TG(42:1); TG(44:1); TG(52:3); PC(16:0/20:4); PC(18:0/18:1); Methyl linoleate; LysoPE(0:0/16:0); LysoPE(0:0/18:1(9Z)); PS(15:0/22:0); PIP(16:1(9Z)/18:0); Stearoyllactic acid; ubiquinone-8; DG(34:1e); LysoPI(20:4); PA(34:1); PC(34:0); PC(40:2e); PS(18:0/22:6); Cholesterol sulfate; SM(d18:1/15:0); Sorbitan stearate; Avenoleic acid; PI(18:0/18:2); PS(16:0/20:4); PI(16:0/18:2); LysoPE(20:1); LysoPA(22:4); DG(40:2); PE(34:3); Glutarate semialdehyde; PC(18:0/16:0); PGP(16:0/18:0); PC(38:7); PC(34:2); LysoPC(P-18:0/0:0); MG(0:0/18:2(9Z,12Z)/0:0); LysoPE(20:1(11Z)/0:0); Octadecanol; PI(16:0/20:2(11Z,14Z)); PE(32:1); Indoxyl glucuronide; PC(18:1); PC(18:0/18:0); TG(51:3); PG(17:0/17:0); Tetracosanoic acid; LysoPA(0:0/16:0); PE(20:0e/22:6); PC(14:0/16:1(9Z)); PA(10:0/19:0); Hexyl heptanoate; LysoPS(18:0/0:0); Palmitoyl Serinol; 3-Hexenoic acid; MG(0:0/18:1(11Z)/0:0); TG(18:4/18:4/22:5); Perillic acid; DG(10:0/a-25:0/0:0); LysoPI(18:1(9Z)/0:0); TG(24:0/24:0/24:0); MG(O-18:0/0:0/0:0); DG(20:0/22:0/0:0); Linalyl anthranilate; PGP(16:1(9Z)/16:1(9Z)); CE(15M5); 7-Sulfocholic acid; LysoPA(21:0/0:0); PA(16:0/18:2(9Z,12Z)); PC(14:0/P-18:0); PGP(a-13:0/i-22:0); Rosmic acid; Carnosic acid; PA(10:0/8:0); LysoPS(18:1(9Z)/0:0); Sciadonic acid; Fusidic Acid; LysoPG(16:0/0:0); Thujyl alcohol; LysoPC(18:1(11Z)/0:0); CL(18:0/18:0/18:0/18:0); 9,10,13-TriHOME; DG(15:0/0:0/16:1n7); CPA(18:1(11Z)/0:0); PI(16:0/22:6); PC(16:1e/20:4); PC(40:7e); PE(18:0e/18:2); PS(18:0/22:4); Senecioic acid; PE(16:0p/22:5); TG(16:0/18:1/18:2); PI(18:0/20:4); PC(33:0); PG(38:6); SM(d42:3); Hex2Cer(d18:1/24:1); Prenyl glucoside; Cer(d18:0/22:0); LysoPE(18:2e); CerG2GNAc1(d36:1); PE(20:0e/18:2); DG(32:1e); MG(0:0/14:1(9Z)/0:0); LysoPE(14:0/0:0); Pubesenolide; Bisnorcholic acid; Dehydrocyanaropicrin; PE(42:9); Trilobinol; 25-Hydroxytachysterol3; CerP(d18:1/12:0); Palmitaldehyde; PS(33:3); 2-Nonenoic acid; (2'E,4'Z,7'Z,8E)-Colnelenic acid; DG(32:1); Norecasantalic acid; LysoPA(18:0E); DG(15:0/0:0/20:4n3); LysoPC(22:5n6); PA(40:8); TG(16:0/18:2/18:2); CerG2GNAc1(d36:2); TG(62:7e); DG(30:3e); TG(16:0/8:0/18:1); MePC(41:4e); TG(70:4); DG(35:1e); ChE(18:1); DG(33:1e); TG(17:0/18:1/18:1); TG(28:0/18:1/24:2); TG(16:0/16:1/18:2); PC(20:0e/22:5); TG(20:0e/18:1/22:6); DG(33:0e); TG(18:1/18:2/20:4); TG(16:0/17:1/18:1); DG(34:5e); TG(28:1/16:0/24:2); TG(19:1/16:0/18:2); PE(42:5e); PIP(52:1); TG(16:1/16:1/18:2); TG(16:0/18:1/22:6); TG(16:0/14:4/16:0); TG(16:0/18:1/18:3); TG(18:0/17:0/18:3); TG(16:0/18:1/18:1); DG(36:5e); TG(18:1/18:1/18:2); PC(40:3e); CerG2GNAc1(d34:2); LysoPE(16:2e); PI(17:0/20:3); PC(36:0e); CerG2GNAc1(d34:1); LysoPC(13:0); TG(19:0/19:0/20:3); PS(20:5/18:2); TG(18:1/18:1/18:1); SM(d39:0); PE(20:1e); PC(38:2e); PE(20:1e/18:2); BiotinylPE(16:4); TG(20:0e/16:0/18:1); PC(42:5e); PE(20:0p/22:5); MePC(14:0e); SM(d18:1/24:2); SM(d43:3); SM(d43:4); PE(20:0p/18:2); PE(18:0p/20:1); PE(16:0/22:5); SM(d41:0); LysoPE(20:0e); LysoPC(18:2e); PC(20:0e/22:6); LdMePE(18:0); PC(42:6e); CL(20:5/18:1/20:1/18:2); PC(20:0e/20:4); PE(16:0/20:5); PC(33:3); PC(36:2e); PA(15:0/20:5); DG(22:4n6/0:0/22:6n3); CL(52:0); AS 1-1; Cheirotoxol; MG(i-17:0/0:0/0:0); TG(72:3); 4-Hydroxyestradiol; Nonyl octanoate; PG(i-13:0/a-15:0); Decyl acetate; PE(14:1/20:5); PA(44:7); Armillaramide; Arjunolic acid; PA(32:5); TG(20:2/22:1/22:6); LysoPG(18:2(9Z,12Z)); PA(22:5/24:1); PA(8:0/14:0); CE(19:0); DG(34:0); Pentyl heptanoate; Epilubimin; FAHFA(16:1(9Z)/5-O-18:0); Pipecuronium; Camelledionol; PA(39:1); Pisumic acid; 1-a,24R,25-Trihydroxyvitamin D2; 15-Epi-lipoxin A4; LysoPC(p-16:0/0:0); 3beta-3-Lupanol; Sterol; Prednisone; LysoPE(20:0/0:0); PA(42:1); Methyl dihydrophaseate; alpha-Peroxyachifolide; 2-Ethylsuberic acid; alpha-Irone; PC(20:5/P-18:1); PGP(16:0/16:1(9Z)); PA(35:2); DG(40:4); Isodigeranyl; PA(8:0/i-15:0); LysoPE(0:0/18:4(6Z,9Z,12Z,15Z)); PS(42:7); Hex1Cer(d19:1/22:6); Cer(t18:0/24:3); Cer(d18:1/24:3); PS(18:2/18:2); CL(18:3/18:2/18:1/16:0); OAHFA(47:4); PE(16:0p/20:4); PE(18:2e/20:4); PC(36:0); PE(18:0e/20:4); DG(16:0/16:0); CL(18:3/20:4/20:4/18:2); PE(18:1p/22:6); PE(20:4/21:1); PC(20:1/14:3); PS(17:1/20:2); PG(19:1/16:0); TG(19:1/17:1/19:1); PE(17:0/20:4); PE(18:1e/22:4); PIP(33:1); PC(18:0e/20:4); MGDG(18:0/20:3); DG(18:0/16:0); PC(35:0); TG(18:0/16:0/18:0); PE(18:0p/22:6); SM(d35:1); MePC(34:4e); Cer(d18:2/24:2); PC(18:1e/20:4); PS(42:7e); CL(18:2/16:0/16:0/18:1); PC(16:0e/20:4); Cer(d36:4); PE(40:9); PI(18:0/16:1); PE(19:1/20:4); TG(18:0/18:0/20:3); PC(29:0); BiotinylPE(27:3); PA(24:2/22:6); PI(17:0/20:4); PE(20:0p/20:4); PC(40:0); Cer(d29:0); TG(18:0/18:0/18:0); PS(18:0/20:3); DG(36:2e); PS(40:7e); MePC(32:5e); TG(25:0/16:0/16:0); PC(31:0); PE(20:0/18:1); Cer(d30:3); Cer(d30:0); TG(16:0e/16:0/18:0); TG(18:0/18:0/19:0); PS(18:0/22:5); MePC(34:5e); TG(12:0/10:3/10:4); PI(18:0/22:6); Cer(t20:0/26:0); SM(d36:1); TG(15:0/16:0/18:3); PS(20:0/22:6); Cer(t18:0/18:0+O); PS(18:0/18:2); TG(15:0/14:0/16:0); Cer(t18:0/20:0+O); DG(39:2); PE(18:2e/20:3); Hex2Cer(d42:1); AEA(16:0); LBPA(16:0/18:2); Hex2Cer(d41:1); PC(15:0/20:4); PI(34:2); Cer(d16:0/20:0+O); CerG2GNAc1(d43:2); Cer(m36:3+2O); PI(18:3e/18:2); PE(18:0p/20:4); PE(41:4e); PS(20:4/20:4); TG(18:0e/11:3/11:3); PS(18:0/20:4); PC(20:0/20:3); AcCa(24:0); LysoSM(d18:0); PIP(40:9e); PS(16:0/22:6); PG(16:0/20:2); Hex2Cer(d42:2); Hex2Cer(d40:1); PG(16:0/18:3); AcCa(20:4); DG(38:5e); SM(d44:6); Cer(d20:0/24:0); BiotinylPE(34:9); PE(20:4e/17:1); SM(t34:1); PA(42:5e); LBPA(16:0/20:4); phSM(d16:0/18:1); PG(16:0/18:1); DG(38:4e); PIP(42:9e); BiotinylPE(36:9); PC(38:8e); TG(16:1/13:0/18:2); Cer(d38:2); PIP2(18:2/22:6); PIP2(20:2/18:2); AEA(18:0); MePC(37:4e); AcCa(20:3); DG(39:7); PC(42:9e); PG(34:2); AcCa(22:6); TG(18:1e/16:0/20:4); AcCa(17:1); AcCa(24:2); PIP2(18:2/20:4); AcCa(18:2); PIP(16:0/10:1); AcCa(10:3); AcCa(20:2); AcCa(22:5); AcCa(22:4); Cer(d36:3+O); PIP(8:0/20:3); PIP(10:0/20:3); AcCa(20:5); PIP(16:0/12:3);