Study M1048
Study name | Radford-Smith DE 2022 |
Title | Modifying the maternal microbiota alters the gut-brain metabolome and prevents emotional dysfunction in the adult offspring of obese dams |
Overall design | The aim of this study was to test whether perinatal intake of a multispecies probiotic could mitigate the abnormal emotional behavior in the juvenile and adult offspring of obese dams. Female CD-1 mice were fed a high-fat or normal diet for 12 weeks, prior to the start of pregnancy until offspring were of weaning age (postnatal day [PND]21). Probiotic supplementation began at pregnancy and continued until PND21. Offspring CD-1 mice were divided into the following 8 groups: (1) juvenile control group (juvenile offspring, normal diet without probiotic treatment), (2) juvenile high-fat diet group (juvenile offspring, high-fat diet without probiotic treatment), (3) juvenile probiotic treatment group (juvenile offspring, normal diet with probiotic treatment), (4) juvenile high-fat diet + probiotic group (juvenile offspring, high-fat diet with probiotic treatment), (5) adult control group (adult offspring, normal diet without probiotic treatment), (6) adult high-fat diet group (adult offspring, high-fat diet without probiotic treatment), (7) adult probiotic treatment group (adult offspring, normal diet with probiotic treatment), and (8) adult high-fat diet + probiotic group (adult offspring, high-fat diet with probiotic treatment). Forced swimming test was performed for both juvenile (PND21) and adult (PND112) offspring. Obese dams exhibited increased depression-like behavior, and probiotic treatment reduced time immobile in the adult offspring. The faece, liver, plasma, and brain metabolome of juvenile (PND21) and adult (PND112) were compared using NMR-based metabolomics technique. |
Study Type | Type1; Type2; Type3; |
Data available | |
Organism | Mouse; CD-1 mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | 191 |
Tissue | Central; Brain; Brain; Peripheral; Liver; Liver; Peripheral; Faece; Faece; Peripheral; Blood; Plasma; |
Platform | NMR; NMR: 700 MHz Bruker AVII spectrometer; |
PMID | |
DOI | |
Citation | Radford-Smith DE, Probert F, Burnet PWJ, et al. Modifying the maternal microbiota alters the gut-brain metabolome and prevents emotional dysfunction in the adult offspring of obese dams. Proc Natl Acad Sci U S A. 2022 Mar 1;119(9):e2108581119. |
Metabolite | Gamma-Aminobutyric acid; L-Leucine; L-Glutamic acid; L-Lactic acid; L-Alanine; L-Glutamine; |
