Study M1015

Study name

Lu C 2022

Title

Soy isoflavones alleviate lipopolysaccharide-induced depressive-like behavior by suppressing neuroinflammation, mediating tryptophan metabolism and promoting synaptic plasticity

Overall design

The aim of this study was to evaluate the antidepressant-like action of soy isoflavones in acute lipopolysaccharide-treated mice and to explore its underlying mechanisms. C57BL/6J mice were divided into the following 6 groups (n = 10 in each group): (1) control group, (2) lipopolysaccharide group, (3) lipopolysaccharide + low dose of soy isoflavones group (lipopolysaccharide injection, with soy isoflavones treatment at the dose of 10 mg/kg), (4) lipopolysaccharide + middle dose of soy isoflavones group (lipopolysaccharide injection, with soy isoflavones treatment at the dose of 20 mg/kg), (5) lipopolysaccharide + high dose of soy isoflavones group (lipopolysaccharide injection, with soy isoflavones treatment at the dose of 40 mg/kg), and (6) lipopolysaccharide + amitriptyline group (lipopolysaccharide injection, with amitriptyline treatment at the dose of 10 mg/kg). Drugs were orally pre-administered to the mice once daily for 14 consecutive days, then a dose of lipopolysaccharide (1 mg/kg) was intraperitoneally injected to induce depressive-like behavior in animals by activating neuroinflammation. The levels of the tryptophan metabolites (5-hydroxytryptamine, tryptophan and kynurenine) in the hippocampus and cortex were detected via LC-MS/MS analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model;

Criteria for depression

Sucrose preference test, forced swimming test, tail suspension test

Sample size

60

Tissue

Central; Brain; Cortex;

Central; Brain; Hippocampus;

Platform

MS-based; LC-MS: SHIMADZU Prominence LC system (Kyoto, Japan) with QTRAP 5500 mass spectrometer (AB SCIEX, Foster City, CA, USA);

PMID

35993820

DOI

10.1039/d2fo01437h

Citation

Lu C, Wei Z, Wang Y, et al. Soy isoflavones alleviate lipopolysaccharide-induced depressive-like behavior by suppressing neuroinflammation, mediating tryptophan metabolism and promoting synaptic plasticity. Food Funct. 2022 Sep 22;13(18):9513-9522.

Metabolite

L-Tryptophan;

L-Kynurenine;

Serotonin;