Study M078
Study name | Gong MJ 2016 |
Title | Icariin reverses corticosterone-induced depression-like behavior, decrease in hippocampal brain-derived neurotrophic factor (BDNF) and metabolic network disturbances revealed by NMR-based metabonomics in rat |
Overall design | This study aimed to investigate antidepressant-like effect and the possible mechanisms of icariin in a rat model of corticosterone (CORT)-induced depression by using a combination of behavioral and biochemical assessments and NMR-based metabonomics. The depression model was established by subcutaneous injections of CORT for 21 consecutive days in rats, as evidenced by reduced sucrose intake and hippocampal brainderived neurotrophic factor (BDNF) levels, together with an increase in immobility time in a forced swim test (FST). Metabonomic analysis identified potential biomarkers associated with depression in serum, urine and brain tissue extract, respectively. Rats were randomly divided into 3 groups (n = 12 in each group): (1) control group (vehicle), (2) CORT model group (CORT plus vehicle), and (3) CORT model + icariin group (CORT plus icariin at the dose of 60 mg/kg/d). Six samples per group were used for metabonomic analysis. Rats in the model and icariin treatment groups were subcutaneously injected with corticosterone (40 mg/kg, once daily) for 3 weeks, and drugs were administered by gastric instillation during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 18 |
Tissue | Peripheral; Blood; Serum; Peripheral; Urine; Urine; Central; Brain; Brain; |
Platform | NMR; NMR: Bruker AVANCE III 500 MHz spectrometer (BrukerBiospin, Rheinstetten, Germany); |
PMID | |
DOI | |
Citation | Gong MJ, Han B, Wang SM, et al. Icariin reverses corticosterone-induced depression-like behavior, decrease in hippocampal brain-derived neurotrophic factor (BDNF) and metabolic network disturbances revealed by NMR-based metabonomics in rat. J Pharm Biomed Anal 2016;123:63-73. |
Metabolite | N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Isoleucine; L-Aspartic acid; Succinic acid; D-Glucose; Phenylacetylglycine; Oxoglutaric acid; Taurine; N-acetyl glycoprotein; |
