Study M069
Study name | Zhai XJ 2015a |
Title | A simple LC-MS/MS method for quantitative analysis of underivatized neurotransmitters in rats urine: assay development, validation and application in the CUMS rat model |
Overall design | To better understand depression, an analytical LC-MS/MS method for the simultaneous determination of 11 underivatized neurotransmitters in a single analytical run was developed. The advantage of this method is the simple preparation in that there is no need to deconjugate the urine samples. Rats were randomly divided into 2 groups (n = 12 in each group): (1) control group, (2) CUMS group (chronic unpredictable mild stress). The CUMS stress procedure lasted for 4 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 24 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Shimadzu LC-30 AD pump (Shimadzu, Kyoto, Japan) with API QTRAP 5500 triple quadrupole mass spectrometer; |
PMID | |
DOI | |
Citation | Zhai XJ, Chen F, Zhu CR, et al. A simple LC-MS/MS method for quantitative analysis of underivatized neurotransmitters in rats urine: assay development, validation and application in the CUMS rat model. Biomed Chromatogr 2015; 29(11): 1737-43. |
Metabolite |
