Study M061
Study name | Liu XY 2015 |
Title | Discovery and validation of plasma biomarkers for major depressive disorder classification based on liquid chromatography-mass spectrometry |
Overall design | In this study, a nontargeted metabolomics approach based on ultra-high-performance liquid chromatography equipped with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to find the differential metabolites in plasma samples from patients with major depressive disorder (MDD) and healthy controls. Furthermore, a validation analysis focusing on the differential metabolites was performed in another batch of samples using a LC-MS-based targeted approach based on the dynamic multiple reactions monitoring method. In the discovery set, first-episode drug-naive MDD patients (depressed group, n = 60) and healthy control subjects (control group, n = 59) were included. In the validation set, the other 75 MDD patients and 52 healthy control subjects were assigned. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Human; |
Categories of depression | Depressive disorder; Depression; Depression; |
Criteria for depression | DSM-IV diagnosed MDD, HAMD-17 > 18 |
Sample size | 246 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Waters ACQUITY ultra-performance liquid chromatography system (UPLC) (Waters, Milford, MA) coupled to an AB SCIEX Triple TOF 5600 System (AB SCIEX, Framingham, MA); MS-based; LC-MS: Shimadzu LC (30AD)-MS (TQ 8050) (Shimadzu, Kyoto, Japan); |
PMID | |
DOI | |
Citation | Liu X, Zheng P, Zhao X, et al. Discovery and validation of plasma biomarkers for major depressive disorder classification based on liquid chromatography-mass spectrometry. J Proteome Res 2015;14(5):2322-30. |
Metabolite | L-Tryptophan; L-Methionine; LysoPC(16:0); Propionylcarnitine; LysoPC(18:1); LysoPC(20:1); LysoPC(P-16:0); LysoPE(18:2); LysoPE(18:1); LysoPC(22:4); LysoPE(20:3); LysoPE(22:5); PE(34:2); PE(36:4); LysoPC(16:1/0:0); LysoPE(16:1); LysoPE(16:0); LysoPE(18:0); PC(32:0); Glycoursodeoxycholic acid; Deoxycholic acid glycine conjugate; Taurochenodesoxycholic acid; 7Z,10Z-Hexadecadienoic acid; 2-trans,4-cis-Decadienoylcarnitine; Hexanoylcarnitine; 9-Decenoylcarnitine; L-Octanoylcarnitine; Decanoylcarnitine; cis-5-Tetradecenoylcarnitine; 3, 5-Tetradecadiencarnitine; Dodecanoylcarnitine; trans-2-Dodecenoylcarnitine; Octadecanamide; Palmitic amide; |
