Study M051
Study name | Bai SJ 2015 |
Title | 1H NMR-based metabolic profiling reveals the effects of fluoxetine on lipid and amino acid metabolism in astrocytes |
Overall design | In this study, primary astrocytes were exposed to 10 uM fluoxetine; 24 h post-treatment, a high-resolution proton nuclear magnetic resonance (1H NMR)-based metabolomic approach coupled with multivariate statistical analysis was used to characterize the metabolic variations of intracellular metabolites. Twelve dishes were exposed to fluoxetine (fluoxetine group) with a concentration of 10 uM, a highly effective concentration selected by the proliferation assay. The remaining 12 control dishes were treated with only serum-free DMEM (control group). |
Study Type | Type3; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 24 |
Tissue | Central; Brain; Astrocyte; |
Platform | NMR; NMR: Varian Unity INOVA 600 NMR spectrometer (Varian Inc., Palo Alto, CA, USA); |
PMID | |
DOI | |
Citation | Bai S, Zhou C, Cheng P, et al. 1H NMR-based metabolic profiling reveals the effects of fluoxetine on lipid and amino acid metabolism in astrocytes. Int J Mol Sci 2015;16(4):8490-504. |
Metabolite | L-Isoleucine; L-Methionine; L-Asparagine; L-Lysine; L-Tyrosine; Ethanolamine; Creatine; Beta-D-Glucose; |
