Study M032
Study name | Tian JS 2013a |
Title | 1H-NMR-based metabonomic studies on the anti-depressant effect of genipin in the chronic unpredictable mild stress rat model |
Overall design | The purpose of this work was to investigate the anti-depressant effect of genipin and its mechanisms using 1H-NMR spectroscopy and multivariate data analysis on a chronic unpredictable mild stress (CUMS) rat model. Rat serum and urine were analyzed by nuclear magnetic resonance (NMR)-based metabonomics after oral administration of either genipin or saline for 2 weeks. Rats were randomly divided into 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stress plus venlafaxine treatment at the dose of 50 mg/kg), (4) CUMS + high dose of genipin group (stress plus genipin treatment at the dose of 100 mg/kg), (5) CUMS + middle dose of genipin group (stress plus genipin treatment at the dose of 50 mg/kg), and (6) CUMS + low dose of genipin group (stress plus genipin treatment at the dose of 25 mg/kg). Six samples per group were used for metabonomic analysis. The CUMS procedure was conducted for 4 weeks, and drugs were administered via gastric intubation during the last two weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 36 |
Tissue | Peripheral; Blood; Serum; Peripheral; Urine; Urine; |
Platform | NMR; NMR: Bruker 600-MHz AVANCE III NMR spectrometer (Bruker, Germany); |
PMID | |
DOI | |
Citation | Tian JS, Shi BY, Xiang H, et al. 1H-NMR-based metabonomic studies on the anti-depressant effect of genipin in the chronic unpredictable mild stress rat model. PLoS One 2013;8(9):e75721. |
Metabolite | Citric acid; 3-Hydroxybutyric acid; L-Lactic acid; |
