Study M009
Study name | Gao XX 2011 |
Title | Metabonomic study on chronic unpredictable mild stress and intervention effects of Xiaoyaosan in rats using gas chromatography coupled with mass spectrometry |
Overall design | This paper was designed to study metabonomic characters of the depression induced by chronic unpredictable mild stress (CUMS) and the therapeutic effects of Xiaoyaosan (XYS) in treating the depression. A plasma metabonomics method based on gas chromatography/mass spectrometry (GC/MS) was developed. Rats were divided into the following 6 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 25 mg/kg), (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 6 mg/kg), (5) CUMS + middle dose of XYS group (stressor plus XYS treatment at the dose of 46.2 g/kg), and (6) CUMS + high dose of XYS group (stressor plus XYS treatment at the dose of 92.4 g/kg). Six samples per group were used for metabonomic analysis. The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 36 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; GC-MS: Polaris Q ion trap mass spectrometer (Thermo Fisher Scientific Inc., USA); |
PMID | |
DOI | |
Citation | Gao X, Zheng X, Li Z, et al. Metabonomic study on chronic unpredictable mild stress and intervention effects of Xiaoyaosan in rats using gas chromatography coupled with mass spectrometry. J Ethnopharmacol 2011;137(1):690-9. |
Metabolite | Linoleic acid; Stearic acid; Palmitic acid; L-Tryptophan; |
