Study M997
Study name | Wang DN 2022 |
Title | Tryptophan-rich diet ameliorates chronic unpredictable mild stress induced depression- and anxiety-like behavior in mice: the potential involvement of gut-brain axis |
Overall design | In this study, a chronic unpredictable mild stress (CUMS)-induced mouse model with depression- and anxiety-like behaviors was used to evaluate the potential effects of tryptophan treatment on the depression, despair, and anxiety-like behavioral disorders. C57BL/6 mice were divided into the following 4 groups: (1) control group (no stress, with normal diet), (2) CUMS group (stressor, with normal diet), (3) tryptophan supplementation group (no stress, with tryptophan supplementation diet), and (4) CUMS + tryptophan supplementation group (stressor, with tryptophan supplementation diet). The CUMS stress procedure lasted for 5 weeks, and tryptophan supplementation was administered during the model building period. Targeted tryptophan metabolism in serum and untargeted metabolomics in the brain were detected by LC-MS (n = 6/group). |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Sucrose preference test, tail suspension test |
Sample size | 24 |
Tissue | Peripheral; Blood; Serum; Central; Brain; Brain; |
Platform | MS-based; LC-MS: Waters Acquity UPLC with QToF Premier mass spectrometer (Waters, UK); MS-based; LC-MS: EXIONLC with SCIEX 6500 QTRAP + triple quadrupole mass spectrometer (Sciex); |
PMID | |
DOI | |
Citation | Wang D, Wu J, Zhu P, et al. Tryptophan-rich diet ameliorates chronic unpredictable mild stress induced depression- and anxiety-like behavior in mice: the potential involvement of gut-brain axis. Food Res Int. 2022 Jul;157:111289. |
Metabolite |
