Study M991
Study name | Dobos N 2012 |
Title | The role of indoleamine 2,3-dioxygenase in a mouse model of neuroinflammation-induced depression |
Overall design | The aim of this study was to determine whether neuroinflammation-induced increased indoleamine 2,3-dioxygenase (IDO) levels in the mammalian brain will lead to depressive-like behavior. Neuroinflammation was initiated in mice by a single intracerebroventricular injection of lipopolysaccharide. 1-Methyl-tryptophan, a competitive IDO-inhibitor, were used to assess the development of depressive-like behavioral symptoms in parallel with IDO expression and activity. C57Bl/6J mice were divided into the following 4 groups (n = 8-10 in each group): (1) control group, (2) lipopolysaccharide group, (3) 1-methyl-tryptophan group, and (4) lipopolysaccharide + 1-methyl-tryptophan group. Lipopolysaccharide (5ug) was intracerebroventricularly injected, and 1-methyl-tryptophan was applied via a slow release pellet. Tryptophan and kynurenine concentrations in serum were determined by XLC-MS/MS, and the ratio of kynurenine and tryptophan were used as an index of IDO activity. |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Mouse; C57Bl/6J mouse; |
Categories of depression | Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | 34 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Dobos N, de Vries EF, Kema IP, et al. The role of indoleamine 2,3-dioxygenase in a mouse model of neuroinflammation-induced depression. J Alzheimers Dis. 2012;28(4):905-15. |
Metabolite |
