Study M989

Study name

Son H 2018c

Title

Glutamine has antidepressive effects through increments of glutamate and glutamine levels and glutamatergic activity in the medial prefrontal cortex

Overall design

The aim of this study was to investigate whether glutamate and glutamine levels and glutamatergic neuronal activity are altered in the medial prefrontal cortex (mPFC) of a chronic restraint stress-induced depressive animal model. C57BL/6 mice were divided into the following 3 groups: (1) control group (no stress, with normal diet during stress), (2) chronic restraint stress group (chronic restraint stress, with normal diet during stress), and (3) chronic restraint stress + glutamine diet group (chronic restraint stress, with glutamine diet during stress). For chronic restraint stress, mice were repeatedly placed in a restrainer for 2 h/day for 15 consecutive days. Amino acid levels in the mPFC and plasma were measured by liquid chromatography mass spectrometry (n = 6-7/group).

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6 mouse;

Categories of depression

Animal model; Chronic restraint stress model; Chronic restraint stress model;

Criteria for depression

Sucrose preference test, tail suspension test

Sample size

19

Tissue

Peripheral; Blood; Plasma;

Central; Brain; Medial prefrontal cortex;

Platform

MS-based; LC-MS: Agilent 6460 LC-MS/MS system (Agilent, Singapore);

PMID

30266598

DOI

10.1016/j.neuropharm.2018.09.040

Citation

Son H, Baek JH, Go BS, et al. Glutamine has antidepressive effects through increments of glutamate and glutamine levels and glutamatergic activity in the medial prefrontal cortex. Neuropharmacology. 2018 Dec;143:143-152.

Metabolite

L-Glutamic acid;

L-Glutamine;