Study M978
Study name | Tian P 2022 |
Title | [Effect of Rehmanniae Radix on depression-like behavior and hippocampal monoamine neurotransmitters of chronic unpredictable mild stress model rats] |
Overall design | The aim of this study was to investigate the effect of Rehmanniae Radix on depression-like behavior and monoamine neurotransmitters of chronic unpredictable mild stress (CUMS) model rats. Sprague-Dawley rats were divided into the following 5 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 5.4 mg/kg), (4) CUMS + low dose of Rehmanniae Radix group (stressor plus Rehmanniae Radix treatment at the dose of 1.8 g/kg), and (5) CUMS + high dose of Rehmanniae Radix group (stressor plus Rehmanniae Radix treatment at the dose of 7.2 g/kg). The CUMS stress procedure lasted for 9 weeks, and drugs were administered via intragastric during the last 4 weeks of the model building period. Ultra performance liquid chromatography-tandem mass spectrometry was used to detect the contents of 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, norepinephrine, and 3-methoxy-4-hydroxyphenyl glycol in the hippocampus of rats. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 50 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Tian P, Zhang W, Li KY, et al. [Effect of Rehmanniae Radix on depression-like behavior and hippocampal monoamine neurotransmitters of chronic unpredictable mild stress model rats]. Zhongguo Zhong Yao Za Zhi. 2022 Sep;47(17):4691-4697. (Article in Chinese) |
Metabolite | Homovanillic acid; |
