Study M966
Study name | Wu WZ 2023 |
Title | A unique insight for Xiaoyao San exerts antidepressant effects by modulating hippocampal glucose catabolism using stable isotope-resolved metabolomics |
Overall design | In this study, stable isotope-resolved metabolomics was used to explore the pathway of Xiaoyao San to modulate glucose catabolism in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 4 groups (n = 12 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 35 mg/kg), and (4) CUMS + Xiaoyao San group (stressor plus Xiaoyao San treatment at the dose of 21.2 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Seven hippocampal samples per group were used for stable isotope-resolved metabolomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 28 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Thermo DIONEX UltiMate 3000 high performance liquid chromatography coupled with Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific Inc., Bremen, Germany); |
PMID | |
DOI | |
Citation | Wu WZ, Ling-Hu T, Zhao YH, et al. A unique insight for Xiaoyao San exerts antidepressant effects by modulating hippocampal glucose catabolism using stable isotope-resolved metabolomics. J Ethnopharmacol. 2023 Jan 10;300:115702. |
Metabolite |
