Study M952
Study name | Lei L 2022 |
Title | Gut microbiota-mediated metabolic restructuring aggravates emotional deficits after anesthesia/surgery in rats with preoperative stress |
Overall design | The aim of this study was to characterize the changes of microbial composition and specific metabolites after anesthesia/surgery in rats with preoperative stress based on 16S rRNA gene sequencing and non-targeted metabolomics technique. Sprague-Dawley rats were divided into the following 2 groups (n = 8 in each group): (1) control group, and (2) preoperative stress and surgery group. For the preoperative stress and surgery group, pregnant rats were subjected to chronic restraint stress from gestational day 12 to 18 for 3 sessions per day (45 min per session) to build a preoperative stress model. Then male rats were subjected to laparotomy under anesthesia on the postnatal day 35. Six fecal samples per group were used for metabonomic analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 12 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: triple quadrupole-linear ion trap mass spectrometer LC-MS/MS System; |
PMID | |
DOI | |
Citation | Lei L, Ji M, Yang J, et al. Gut microbiota-mediated metabolic restructuring aggravates emotional deficits after anesthesia/surgery in rats with preoperative stress. Front Immunol. 2022 Aug 8;13:819289. |
Metabolite | Suberic acid; N-alpha-Acetyl-L-lysine; L-3-Phenyllactic acid; 2-Aminoadipic acid; |
