Study M951

Study name

Lei CF 2022

Title

Integrating metabolomics and network analysis for exploring the mechanism underlying the antidepressant activity of paeoniflorin in rats with CUMS-induced depression

Overall design

In this study, a combination of network pharmacology and LC-MS-based untargeted metabolomics was employed to explore urinary biomarkers of paeoniflorin in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CUMS group, and (3) CUMS + paeoniflorin group (stressor plus paeoniflorin treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered via intragastric once per day during the last 3 weeks of model building period. A urinary metabolomics method was applied to evaluate the efficacy of paeoniflorin. Eight samples per group were used for metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

24

Tissue

Peripheral; Urine; Urine;

Platform

MS-based; LC-MS: UHPLC-QExactiveHF-X system (Thermo Fisher Scientific);

PMID

35770096

DOI

10.3389/fphar.2022.904190

Citation

Lei C, Chen Z, Fan L, et al. Integrating metabolomics and network analysis for exploring the mechanism underlying the antidepressant activity of paeoniflorin in rats with CUMS-induced depression. Front Pharmacol. 2022 Jun 13;13:904190.

Metabolite

Citric acid;

5-Hydroxyindoleacetic acid;

Thiamine monophosphate;

Sumiki's acid;

Gluconolactone;

Quinone;

Erucamide;

12-oxo-5E,8E,10Z-dodecatrienoic acid;

(+/-)-Hexanoylcarnitine;

Blumenol C glucoside;

D-(+)-dihydrocarvone;

S-Nitrosoglutathione;

Biopterin;

3-carboxy-4-methyl-5-pentyl-2-furanpropanoic acid;

Alanylproline;

Tranylcypromine glucuronide;

(Z)-3-Oxo-2-(2-pentenyl)-1-cyclopenteneacetic acid;

3-Hydroxytetradecanedioic acid;

O-Desmethylvenlafaxine glucuronide;

10-Hydroxymyoporone;

N-Acetyltyramine;

Tsangane L 3-glucoside;

p-Isoamylphenol;

Trigoforin;

5-Nitro-2-(3-phenylpropylamino)benzoic acid;

3,8-Dihydroxy-6-methoxy-7(11)-eremophilen-12,8-olide;

Erysonine;

4-Hydroxy-5-(4-hydroxy-3-methoxyphenyl)pentanoic acid;

2,6-Dioxo-6-phenylhexanoate;

Ethanethioic acid;

(5R)-5-Hydroxyhexanoic acid;

5-Hydroxy-2-(5-methyl-1-oxo-4-hexenyl)benzofuran;

5'-Deoxyadenosine;

4-Hydroxy-5-(phenyl)-valeric acid-O-sulphate;

beta-D-Glucopyranosyl-11-hydroxyjasmonic acid;

Valproic acid glucuronide;

{[1-(4-methoxyphenyl)-4-methylpent-1-en-3-yl]oxy}sulfonic acid;

xi-3-Hydroxy-5-phenylpentanoic acid O-beta-D-Glucopyranoside;

alpha-CEHC glucuronide;

6-(4-ethyl-2-methoxyphenoxy)-3,4,5-trihydroxyoxane-2-carboxylic acid;

1,4-Ipomeadiol;

3,4,5-trihydroxy-6-[(2-hydroxy-2-methylpropanoyl)oxy]oxane-2-carboxylic acid;

Mellein;

1-(Malonylamino)cyclopropanecarboxylic acid;

{[1-(2-hydroxyphenyl)-3-oxopropan-2-yl]oxy}sulfonic acid;

6-{4-[(1E)-3-[(3-carboxy-5,6-dihydroxycyclohex-3-en-1-yl)oxy]-3-oxoprop-1-en-1-yl]-2-hydroxyphenoxy}-3,4,5-trihydroxyoxane-2-carboxylic acid;

2-{4-[(1E)-1,2-diphenylbut-1-en-1-yl]phenoxy}ethan-1-ol;

Dihydromaleimide beta-D-glucoside;

Cis-2,3-Dihydroxy-2,3-dihydro-p-cumate;

Veranisatin A;

2-Methoxyhydroquinone;

N-(2-Hydroxyethyl)iminodiacetic acid;

Stachyose;

9,15-dioxo-11R-hydroxy-2,3,4,5-tetranor-prostan-1,20-dioic acid;

{4-[(E)-2-{3,5-dihydroxy-4-[(1E)-3-methylbuta-1,3-dien-1-yl]phenyl}ethenyl]phenyl}oxidanesulfonic acid;

Trans-Chlorogenic acid;