Study name | Lei CF 2022 |
Title | Integrating metabolomics and network analysis for exploring the mechanism underlying the antidepressant activity of paeoniflorin in rats with CUMS-induced depression |
Overall design | In this study, a combination of network pharmacology and LC-MS-based untargeted metabolomics was employed to explore urinary biomarkers of paeoniflorin in the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CUMS group, and (3) CUMS + paeoniflorin group (stressor plus paeoniflorin treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered via intragastric once per day during the last 3 weeks of model building period. A urinary metabolomics method was applied to evaluate the efficacy of paeoniflorin. Eight samples per group were used for metabonomic analysis. |
Type1; Type2; | |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 24 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: UHPLC-QExactiveHF-X system (Thermo Fisher Scientific); |
PMID | |
DOI | |
Citation | Lei C, Chen Z, Fan L, et al. Integrating metabolomics and network analysis for exploring the mechanism underlying the antidepressant activity of paeoniflorin in rats with CUMS-induced depression. Front Pharmacol. 2022 Jun 13;13:904190. |
Metabolite | 12-oxo-5E,8E,10Z-dodecatrienoic acid; 3-carboxy-4-methyl-5-pentyl-2-furanpropanoic acid; (Z)-3-Oxo-2-(2-pentenyl)-1-cyclopenteneacetic acid; 3-Hydroxytetradecanedioic acid; O-Desmethylvenlafaxine glucuronide; 5-Nitro-2-(3-phenylpropylamino)benzoic acid; 3,8-Dihydroxy-6-methoxy-7(11)-eremophilen-12,8-olide; 4-Hydroxy-5-(4-hydroxy-3-methoxyphenyl)pentanoic acid; 5-Hydroxy-2-(5-methyl-1-oxo-4-hexenyl)benzofuran; 4-Hydroxy-5-(phenyl)-valeric acid-O-sulphate; beta-D-Glucopyranosyl-11-hydroxyjasmonic acid; {[1-(4-methoxyphenyl)-4-methylpent-1-en-3-yl]oxy}sulfonic acid; xi-3-Hydroxy-5-phenylpentanoic acid O-beta-D-Glucopyranoside; 6-(4-ethyl-2-methoxyphenoxy)-3,4,5-trihydroxyoxane-2-carboxylic acid; 3,4,5-trihydroxy-6-[(2-hydroxy-2-methylpropanoyl)oxy]oxane-2-carboxylic acid; 1-(Malonylamino)cyclopropanecarboxylic acid; {[1-(2-hydroxyphenyl)-3-oxopropan-2-yl]oxy}sulfonic acid; 2-{4-[(1E)-1,2-diphenylbut-1-en-1-yl]phenoxy}ethan-1-ol; Dihydromaleimide beta-D-glucoside; Cis-2,3-Dihydroxy-2,3-dihydro-p-cumate; N-(2-Hydroxyethyl)iminodiacetic acid; 9,15-dioxo-11R-hydroxy-2,3,4,5-tetranor-prostan-1,20-dioic acid; |