Study M948
Study name | Huang MH 2022 |
Title | Comprehensive pseudotargeted metabolomics analysis based on two-phase liquid extraction-UHPLC-MS/MS for the investigation of depressive rats |
Overall design | In this study, a comprehensive pseudotargeted metabolomics method based on two-phase liquid extraction using ultra-high-performance liquid chromatography-tandem mass spectrometry was proposed. The established pseudotargeted method was applied to the metabolomics study of the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 2 groups (n = 8 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. Plasma samples were used for metabolomics analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Not reported |
Sample size | 16 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Shimadzu Nexera UHPLC-30AD system and LCMS-8060 mass spectrometer (Shimadzu, Japan); |
PMID | |
DOI | |
Citation | Huang M, Zhou T. Comprehensive pseudotargeted metabolomics analysis based on two-phase liquid extraction-UHPLC-MS/MS for the investigation of depressive rats. J Sep Sci. 2022 Aug;45(15):2977-2986. |
Metabolite | LysoPC(16:0); LysoPC(18:0); Dihydroxyacetone phosphate; 3,4-Dihydroxymandelic acid; LysoPC(18:2); PC(36:4); LysoPE(20:3); LysoPE(18:3); Cholesteryl acetate; LysoPC(18:3); 2,5-Dichloro-4-oxohex-2-enedioate; |
