Study M934
Study name | Wu LJ 2022 |
Title | Oral administration of 5-hydroxytryptophan restores gut microbiota dysbiosis in a mouse model of depression |
Overall design | The aim of this study was to determine the effects of 5-hydroxytryptophan on the mouse gut microbiome. Both the high-fat diet and chronic unpredictable stress (CUS) were used to induce the depression phenotype in mice. C57BL/6J mice were divided into the following 4 groups (n = 8/group): (1) control group (normal diet, no stress), (2) control + 5-hydroxytryptophan group (normal diet, no stress, with 5-hydroxytryptophan treatment at the dose of 100 mg/kg), (3) CUS + high-fat diet group (high-fat diet and stress), and (4) CUS + high-fat diet + 5-hydroxytryptophan group (high-fat diet and stress, with 5-hydroxytryptophan treatment at the dose of 100 mg/kg). For the first 5 weeks, mice received CUS stress or no stress. Then mice were orally gavaged daily with 5-hydroxytryptophan or vehicle for 8 weeks. The concentrations of 11 short-chain fatty acids in the serum samples of mice in the four groups were determined using GC-MS analysis. |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 32 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; GC-MS: Shimadzu GC2030-QP2020 NX gas chromatography-mass spectrometer; |
PMID | |
DOI | |
Citation | Wu L, Ran L, Wu Y, et al. Oral administration of 5-hydroxytryptophan restores gut microbiota dysbiosis in a mouse model of depression. Front Microbiol. 2022 Apr 28;13:864571. |
Metabolite |
