Study M930
Study name | Li D 2022b |
Title | 3beta-Hydroxysteroid dehydrogenase expressed by gut microbes degrades testosterone and is linked to depression in males |
Overall design | The aim of this study was to evaluate the relationships among testosterone deficiency, depression, and the gut microbiota. Patients who met the diagnostic criteria for major depressive disorder or dysthymic disorder and healthy participants were included. Then Sprague-Dawley rats received antibiotic cocktail for fecal microbiota transplantation (FMT). Sprague-Dawley rats were divided into the following 3 groups: (1) no microbiota group (rats received saline), (2) non-depression microbiota group (rats received FMT from healthy participants), and (3) depression microbiota group (rats received FMT from patients with depression). FMT from patients with depression induced depression-like behavior in rats. Serum and brain samples were used for targeted metabonomic analysis (n =6/group). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Tail suspension test, forced swimming test |
Sample size | 18 |
Tissue | Peripheral; Blood; Serum; Central; Brain; Brain; |
Platform | MS-based; LC-MS: Ekspert ultraLC 100-XL system (AB SCIEX, Framingham, MA, USA) with AB SCIEX 4500 QTRAP mass spectrometer; |
PMID | |
DOI | |
Citation | Li D, Liu R, Wang M, et al. 3beta-Hydroxysteroid dehydrogenase expressed by gut microbes degrades testosterone and is linked to depression in males. Cell Host Microbe. 2022 Mar 9;30(3):329-339.e5. |
Metabolite |
