Study M922
Study name | Yan L 2022 |
Title | Platycladus orientalis seed extract as a potential triple reuptake MAO inhibitor rescue depression phenotype through restoring monoamine neurotransmitters |
Overall design | The aim of this study was to investigate the mechanism of action of the seed extracts of P. orientalis (S4) to rescue depressive behavior in a chronic unpredicted mild stress (CUMS)-induced model in rats. Sprague-Dawley rats were divided into the following 5 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg), (4) CUMS + middle dose of P. orientalis group (stressor plus P. orientalis treatment at the dose of 33 mg/kg/d), and (5) CUMS + high dose of P. orientalis group (stressor plus P. orientalis treatment at the dose of 100 mg/kg/d). The CUMS stress procedure lasted for 3 weeks, and drugs were administered via intragastric once per day for 6 weeks (including 3-week pretreatment). The main monoamine neurotransmitters and their metabolite concentrations in the hippocampus, prefrontal cortex and dorsal raphe nucleus were measured by rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (n =5/group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail-suspension test |
Sample size | 25 |
Tissue | Central; Brain; Hippocampus; Central; Brain; Prefrontal cortex; Central; Brain; Dorsal raphe nucleus; |
Platform | MS-based; LC-MS: 1200SL RRLC-6410 Triple Quad Mass Spectrometer (Agilent Technology, Santa Clara, CA, USA); |
PMID | |
DOI | |
Citation | Yan L, Wang J, He X, et al. Platycladus orientalis seed extract as a potential triple reuptake MAO inhibitor rescue depression phenotype through restoring monoamine neurotransmitters. J Ethnopharmacol. 2022 Apr 28:115302. |
Metabolite | Serotonin; Norepinephrine; Dopamine; 3,4-Dihydroxybenzeneacetic acid/Dopamine ratio; |
