Study M919

Study name

Xiao WP 2022a

Title

The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids

Overall design

The aim of this study was to explore the role of short-chain fatty acid (SCFA) metabolism in chronic cerebral hypoperfusion. In the first study, Sprague-Dawley rats were divided into the following 2 groups: (1) sham group, and (2) chronic cerebral hypoperfusion group. Rats subjected to bilateral common carotid artery occlusion as a model of chronic cerebral hypoperfusion exhibited depressive-like behaviors. Hippocampal SCFA concentrations were assessed using GC-MS (n = 6/group). In the second study, fecal microbiota transplantation (FMT) was conducted in antibiotics-treated rats. Rats were divided into the following 4 groups: (1) sham operation + FMT from sham group (rats received FMT from sham rats, then subjected to sham operation), (2) chronic cerebral hypoperfusion + FMT from sham group (rats received FMT from sham rats, then subjected to chronic cerebral hypoperfusion), (3) sham operation + FMT from chronic cerebral hypoperfusion group (rats received FMT from chronic cerebral hypoperfusion rats, then subjected to sham operation), and (4) chronic cerebral hypoperfusion + FMT from chronic cerebral hypoperfusion group (rats received FMT from chronic cerebral hypoperfusion rats, then subjected to chronic cerebral hypoperfusion). Both the chronic cerebral hypoperfusion groups showed an obvious reduction in their preference for sucrose compared with their paired controls. However, this anhedonia was ameliorated by healthy FMT. Hippocampal SCFA concentrations were also assessed using GC-MS (n = 8/group).

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Sucrose preference test, forced swimming test, tail suspension test

Sample size

44

Tissue

Central; Brain; Hippocampus;

Platform

MS-based; GC-MS: 7890B5977B (Agilent Technologies Inc.);

PMID

35430804

DOI

10.1186/s40168-022-01255-6

Citation

Xiao W, Su J, Gao X, et al. The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids. Microbiome. 2022 Apr 17;10(1):62.

Metabolite

Caproic acid;

Acetic acid;