Study M909
Study name | Moaddel R 2022b |
Title | Comparative metabolomic analysis in plasma and cerebrospinal fluid of humans and in plasma and brain of mice following antidepressant-dose ketamine administration |
Overall design | The aim of this study was to determine the contribution of (2 R,6 R)-hydroxynorketamine to changes in the metabolomic profile observed following ketamine treatment. CD-1 mice were divided into the following 3 groups: (1) control group (saline), (2) ketamine group, and (3) hydroxynorketamine group. Mice received a single intraperitoneal (10 ml/kg) injection of saline, 10 mg/kg of ketamine hydrochloride, or 10 mg/kg of (2 R,6 R)-hydroxynorketamine hydrochloride. Samples were collected at baseline (no injection) (n = 8) and at four timepoints post-administration (15 min, 60 min, 240 min, and 24 h) in different cohorts of mice. A targeted metabolomic analysis of kynurenines and nicotinamide adenine dinucleotide was carried out in plasma, hippocampus, and hypothalamus. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; CD-1 mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 72 |
Tissue | Peripheral; Blood; Plasma; Central; Brain; Hippocampus; Central; Brain; Hypothalamus; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Moaddel R, Zanos P, Farmer CA, et al. Comparative metabolomic analysis in plasma and cerebrospinal fluid of humans and in plasma and brain of mice following antidepressant-dose ketamine administration. Transl Psychiatry. 2022 May 2;12(1):179. |
Metabolite | L-Phenylalanine; L-Glutamic acid; Glycine; L-Asparagine; L-Cystine; L-Histidine; Aminoadipic acid; Sarcosine; Cer(d18:1/24:0); CE(16:1); PC aa C36:6; TG(18:0/38:6); HexCer(d18:1/20:0); |
