Study M904

Study name

Jiang WX 2022

Title

Alteration of glycerophospholipid metabolism in hippocampus of post-stroke depression rats

Overall design

The aim of this study was to find out whether the lipid metabolism in the hippocampus is involved in the pathogenesis of post-stroke depression. For the post-stroke depression model, a focal cerebral ischemia and reperfusion rat model was established by transient middle cerebral artery occlusion, then rats were exposed to chronic unpredictable mild stress (CUMS) for 4 weeks. Sprague-Dawley rats were divided into the following 3 groups: (1) control group (sham operation, no stress), (2) stroke group (transient middle cerebral artery occlusion, no stress), and (3) stroke + CUMS group (transient middle cerebral artery occlusion, with four-week stress). Six hippocampal samples per group were used for LC-MS-based metabonomic analysis.

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

18

Tissue

Central; Brain; Hippocampus;

Platform

MS-based; LC-MS: LC with Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher);

PMID

35469367

DOI

10.1007/s11064-022-03596-y

Citation

Jiang W, Chen J, Gong L, et al. Alteration of glycerophospholipid metabolism in hippocampus of post-stroke depression rats. Neurochem Res. 2022 Apr 25.

Metabolite

Coenzyme Q9;

TG(16:0/14:0/22:6);

TG(16:0/16:0/21:5);

TG(16:0/22:6/22:6);

TG(18:0/18:0/22:4);

TG(18:0/22:4/22:6);

TG(18:0/22:6/22:6);

TG(18:1/18:1/22:6);

TG(20:3/22:6/22:6);

CL(16:0/16:1/18:1/20:4);

CL(18:1/16:1/18:1/16:0);

CL(18:1/16:1/18:1/18:1);

CL(18:1/16:1/18:1/20:4);

CL(18:1/18:1/18:1/16:0);

CL(18:1/18:1/18:1/18:1);

CL(18:1/18:1/18:1/18:2);

CL(18:1/18:1/18:1/20:4);

CL(18:2/16:1/22:6/20:4);

CL(18:2/18:1/18:1/22:6);

CL(18:2/18:1/20:4/18:1);

CL(18:2/18:1/20:4/22:6);

CL(18:2/20:3/16:1/18:1);

CL(18:2/20:4/18:1/20:4);

CL(20:4/16:0/20:4/18:1);

CL(20:4/18:1/18:1/16:0);

CL(20:4/18:1/20:3/18:1);

CL(20:4/20:4/18:1/22:6);

CL(22:3/18:0/18:0/20:4);

CL(22:6/18:1/16:0/18:1);

CL(22:6/18:1/18:1/20:4);

CL(22:6/18:1/18:1/22:6);

CL(22:6/20:4/20:4/20:4);

CL(83:8);

PC(16:0/22:4);

PC(16:0p/22:6);

PC(20:1/20:4);

PC(33:0);

PE(18:0p/24:1);

PE(39:1p);

PE(40:0e);

PE(51:8);

PEt(42:4p);

PEt(44:8);

PG(38:6);

PMe(45:8);

PS(41:3);

Cer(d28:0);

CerG2(d27:0);

SM(d20:1/16:0);

So(d18:0);