Study M849
Study name | Zhang R 2021a |
Title | A reliable high-throughput screening model for antidepressant |
Overall design | In this study, the authors established the chronic unpredictable mild stress (CUMS) model and reserpine model in mice and zebrafish, respectively. The effects of CUMS and reserpine were preliminarily analyzed through behavioral tests and brain pathology sections, and then compared metabolic differences through metabolomics. C57BL/6 mice were divided into the following 2 groups: (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 7 weeks. Eight brain samples per group were used for metabonomic analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail suspension test |
Sample size | 16 |
Tissue | Central; Brain; Brain; |
Platform | MS-based; LC-MS: Agilent 1260 high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer (Agilent, Santa Clara, CA, USA); |
PMID | |
DOI | |
Citation | Zhang R, Qiao C, Liu Q, et al. A reliable high-throughput screening model for antidepressant. Int J Mol Sci. 2021 Sep 1;22(17):9505. |
Metabolite | L-Glutamic acid; Succinic acid; Phenylpyruvic acid; Oxoglutaric acid; cis-Aconitic acid; Inosine; 1-Methylnicotinamide; Glutathione; Allantoin; O-Phosphoethanolamine; Sphingosine 1-phosphate; Pyridoxamine; 4-Hydroxyphenylpyruvic acid; N-Acetylaspartylglutamic acid; 3-Sulfinoalanine; Formiminoglutamic acid; 6-Hydroxymelatonin; N-Formyl-L-aspartate; Flavin mononucleotide; 2-Hydroxyestrone; 5-Aminoimidazole ribonucleotide; N-Glycolylneuraminic acid; Paraxanthine; CMP-N-trimethyl-2-aminoethylphosphonate; |
