Study M829

Study name

Cong MY 2021

Title

[Antidepressant mechanism of Shugan Lipi recipe in regulating tryptophan metabolism and its common pharmacodynamic substances]

Overall design

The aim of this study was to explore the antidepressant mechanism of Shugan Lipi recipe in regulating tryptophan metabolism, and to find out their common pharmacodynamic substances in a chronic unpredictable mild stress (CUMS) model of depression. C57BL/6J mice were divided into the following 6 groups: (1) control group, (2) CUMS group, (3) CUMS + Chaihu Shugan Powder group (stressor plus Chaihu Shugan Powder treatment at the dose of 41 g/kg), (4) CUMS + Sini Powder group (stressor plus Sini Powder treatment at the dose of 31 g/kg), (5) CUMS + Xiaoyao Powder group (stressor plus Xiaoyao Powder treatment at the dose of 133 g/kg), and (6) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 7.8 mg/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered via intragastric once per day during the last 15 days of the model building period. LC-MS/MS method was used to determine the contents of 5-hydroxytryptamine, kynurenine and tryptophan in liver, brain, colon and other tissues. Five samples per group were used for targeted metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test, tail suspension test

Sample size

30

Tissue

Peripheral; Gut; Colon;

Peripheral; Liver; Liver;

Central; Brain; Brain;

Platform

MS-based; LC-MS: 1260 HPLC system with 6460 triple quadruple mass spectrometer (Agilent);

PMID

34402287

DOI

10.19540/j.cnki.cjcmm.20210120.402

Citation

Cong MY, Liang XX, Chen FL, et al. [Antidepressant mechanism of Shugan Lipi recipe in regulating tryptophan metabolism and its common pharmacodynamic substances]. Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(14):3633-3642. (Article in Chinese)

Metabolite

Kynurenine/Tryptophan ratio;

Serotonin/Tryptophan ratio;