Study M795
Study name | Pan CQ 2021 |
Title | Depression accelerates gastric cancer invasion and metastasis by inducing a neuroendocrine phenotype via the catecholamine/beta2 -AR/MACC1 axis |
Overall design | The aim of this study was to determine the effects of depression on gastric cancer progression. BALB/c nude mice were used to establish a model of depression caused by chronic unpredictable mild stress (CUMS). A lung metastasis mouse model was constructed via tail vein injection, and the CUMS depression mouse model was used to explore the role of beta2-AR in depression-induced gastric cancer invasion and metastasis. BALB/c nude mice were divided into the following 4 groups (n =10/group): (1) gastric cancer group, (2) gastric cancer + CUMS group, (3) gastric cancer + ICI-118,551 group, and (4) gastric cancer + ICI-118,551+ CUMS group. At the 3rd week after CUMS treatment, all mice were severally injected with 3 x 10^6 MKN45 cells to establish the gastric cancer model. Next, mice received PBS or ICI-118,551 (5 mg/kg) by daily intraperitoneal injections for 4 weeks. Mice in the two stressed groups exhibited depression-like behaviors. Plasma catecholamine levels were examined by high performance liquid chromatography coupled with tandem mass spectrometry. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; BALB/c nude mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail suspension test |
Sample size | 40 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: 4000 Q TRAP LC/MS/MS system (Applied Biosystems, Foster City, CA, USA); |
PMID | |
DOI | |
Citation | Pan C, Wu J, Zheng S, et al. Depression accelerates gastric cancer invasion and metastasis by inducing a neuroendocrine phenotype via the catecholamine/beta2 -AR/MACC1 axis. Cancer Commun. 2021 Jul 20. |
Metabolite |
