Study M705
Study name | Portero-Tresserra M 2018 |
Title | Maternal separation increases alcohol-drinking behaviour and reduces endocannabinoid levels in the mouse striatum and prefrontal cortex |
Overall design | The aim of this study was to evaluate the long-term effects of maternal separation with early weaning (MSEW) on emotional and social behaviour, alcohol rewarding properties, and alcohol consumption, abstinence and relapse in adolescent male C57BL/6 mice. C57BL/6 mice were divided into the following 2 groups: (1) control group (standard nest), and (2) maternal separation group (maternal separation with early weaning). Endocannabinoids and related compounds were quantified in plasma, striatum and prefrontal cortex by liquid chromatography-tandem mass spectrometry (n = 9-10). Monoamine compounds and corticosteroids were determined by LC-MS/MS (n = 7-8). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Tail suspension test |
Sample size | 19 |
Tissue | Central; Brain; Striatum; Central; Brain; Prefrontal cortex; Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Agilent 6410 triple quadrupole (Agilent Technologies, Wilmington, DE); Agilent 6410 triple quadrupole (Agilent Technologies, Wilmington, DE) (Waters Associates, Milford, MA, USA); |
PMID | |
DOI | |
Citation | Portero-Tresserra M, Gracia-Rubio I, Cantacorps L, et al. Maternal separation increases alcohol-drinking behaviour and reduces endocannabinoid levels in the mouse striatum and prefrontal cortex. Eur Neuropsychopharmacol. 2018;28(4):499-512. |
Metabolite | L-Tyrosine; 5-Hydroxyindoleacetic acid; MG(0:0/20:4(5Z,8Z,11Z,14Z)/0:0); Anandamide; MG(0:0/18:1(9Z)/0:0); |
