Study M700

Study name	Chen JJ 2019
Title	Absence of gut microbiota affects lipid metabolism in the prefrontal cortex of mice
Overall design	In this study, germ-free (GF) mice, specific pathogen-free (SPF) mice and colonized GF (CGF) mice were used to study the effects of gut microbiota on the lipid metabolism in the prefrontal cortex of mice. Balb/c mice were divided into the following 3 groups (n = 8 in each group): (1) control group (specific pathogen-free), (2) germ-free group, and (3) colonized germ-free group (germ-free mice placed into the cages with bedding and fecal matter from SPF mice). GF and CGF mice displayed a decreased proportion of immobility time relative to SPF mice.
Study Type	Type1;
Data available	Unavailable
Organism	Mouse; Balb/c mouse;
Categories of depression	Animal model; Other animal model; Other animal model;
Criteria for depression	Forced swimming test
Sample size	24
Tissue	Central; Brain; Prefrontal cortex;
Platform	MS-based; LC-MS: UHPLC (1290 Infinity LC, Agilent Technologies) coupled to quadrupole time-of-flight (AB Sciex TripleTOF 6600);
PMID	31587617
DOI	10.1080/01616412.2019.1675021
Citation	Chen JJ, Xie J, Zeng BH, et al. Absence of gut microbiota affects lipid metabolism in the prefrontal cortex of mice. Neurol Res. 2019;41(12):1104-1112.
Metabolite	PG(16:0/18:1)-H; PC(38:2)+H; LysoPE(18:0)-H; DG(18:0/22:4)+NH4; PC(42:1)+H; PC(36:0)+H; ChE(22:6)+NH4; Cer(d18:1/18:0)+H; PE(18:0/22:3)+Na; CerG2(d18:1/18:0)+H; TG(18:0/16:0/19:0)+NH4; LysoPC(14:0)+H; LysoPC(16:1)+H; LysoPC(16:1)+Na; PG(36:3)+NH4; PI(18:1/18:1)+Na; PC(40:6)+H; PE(18:0p/20:3)+H; Cer(d18:1/22:1)+H; SM(d42:1)+H; MGDG(18:1/18:1)+Na; MGDG(16:0/18:1)+Na; LysoPC(24:0)+H; CerG1(d18:1/18:1)+H; PC(44:2)+H; LysoPC(22:0)+H; PC(42:2)+H; CerG1(d18:0/18:0)+H; PE(16:0p/20:0)+H; PE(38:1e)+Na; PC(40:0)+H; PC(40:0)+Na; PC(18:1/24:1)+HCOO; PS(47:1)-H; PE(20:0p/20:4)-H; PE(18:0/20:3)-H; PS(38:3)-H; CL(18:1/16:0/16:1/22:6)-H; PE(38:3p)-H; PE(20:0p/22:4)-H; SM(d22:1/20:1)+HCOO; CL(18:2/18:1/16:1/18:1)-H; PS(38:6p)-H; PE(36:0e)-H; GM1(d36:2)-H; PE(18:1/22:6)-H; PE(38:0p)-H; SQDG(16:0/16:0)-H; PE(36:0p)-H; PE(40:1e)-H; PE(40:0e)-H; LysoPG(20:4)-H; PC(20:1/20:4)+HCOO; PI(16:0/22:6)-H; PG(18:1/22:6)-H; PG(34:2p)-H; CL(22:6/16:1/18:1/22:6)-H; PE(16:0p/20:4)-H; LysoPC(16:1)+HCOO; PC(18:0/22:3)+HCOO; FA(20:4)-H; MGDG(16:0/18:1)+HCOO; PE(18:0/20:2)-H; PC(24:1/20:4)+HCOO; PS(38:4p)-H; PE(36:3p)-H; PC(42:4)+Na; PC(54:7)+H; Cer(d20:1/24:0)+H; TG(18:0/16:0/16:0)+NH4; TG(18:1/20:4/22:6)+NH4; PC(56:6)+H; PE(16:1p/22:6)+H; PS(18:0/22:4)+H; LysoPC(32:1)+Na; DG(18:0/20:1)+NH4; TG(18:0p/16:0/20:4)+NH4; LysoPE(18:0)+Na; PE(16:1/20:4)+H; PE(16:1/20:4)+Na; TG(16:0/16:0/18:1)+NH4; PC(36:3)+Na; PI(20:4/20:4)+NH4; LysoPC(22:4)+H; PE(32:0)+H; PC(32:2)+H; PE(16:0p/22:5)+H; TG(16:0/20:4/22:6)+NH4; PE(36:2)+H; PI(38:6)+Na; PC(44:11)+H; TG(18:0/16:0/22:5)+NH4; TG(18:0/18:0/18:0)+NH4; TG(16:0/18:1/22:5)+NH4; PS(22:5/22:6)+H; LysoPE(22:5)+H; PE(18:0/22:5)+H; PE(16:0/22:5)+H; LysoPC(16:0)+H; Co(Q8)+NH4; PE(18:2/22:6)+H; PC(52:5)+H; PG(36:2)+Na; PG(18:1/18:1)+Na; PC(34:2)+H; PC(34:2)+Na; LysoPE(18:0)+H; PE(16:0/20:5)+H; PC(40:5)+H; PC(56:5)+H; PG(20:4/22:6)-H; PS(18:1/22:6)-H; PI(16:0e/20:4)-H; LysoPC(16:0)+HCOO; PG(16:0/18:2)-H; PE(16:0/16:0)-H; CL(20:4/18:0/16:0/18:1)-H; PE(16:0/20:5)-H; PE(16:0p/20:5)-H; PE(16:0/16:1)-H; PE(16:0/18:2)-H; PG(16:1/22:6)-H; PE(16:1/18:2)-H; PE(16:1/20:4)-H; PS(22:4/22:6)-H; CL(18:1/18:1/18:1/16:0)-H; CL(20:4/18:1/18:1/20:4)-H; CL(20:4/16:0/16:0/18:1)-H; Cer(d20:1/18:0)+HCOO; CL(18:1/16:0/16:1/20:4)-H; PE(22:5/22:6)-H; PS(20:3/22:5)-H; CL(20:5/18:1/18:2/20:4)-H; PC(16:1/22:6)+HCOO; MGDG(34:3)+HCOO; PC(22:5/22:6)+HCOO; PE(18:1p/20:4)-H; PE(18:0/22:5)-H; PE(16:0/22:5)-H; LysoPE(22:5)-H; FA(22:5)-H;