Study M698
Study name | Guo JJ 2020 |
Title | Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of Saikosaponin A |
Overall design | The aim of this study was to explore the anti-depression effect of Saikosaponin A and screen the target proteins regulated by Saikosaponin A in a rat model of chronic unpredictable mild stress (CUMS)-induced depression. Sprague-Dawley rats were divided into the following 3 groups (n = 15 in each group): (1) control group, (2) CUMS group, and (3) CUMS + Saikosaponin A group (stressor plus Saikosaponin A treatment at the dose of 50 mg/kg). The CUMS stress procedure lasted for 8 weeks, and drugs were administered via intragastric once per day during the last 4 weeks. 5-Hydroxytryptamine, norepinephrine and dopamine levels in the hippocampus were measured using HPLC-MS (n = 6 per group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 18 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Agilent 1200 series HPLC system (Agilent, USA) with mass spectrometer (Agilent, 6410B, USA); |
PMID | |
DOI | |
Citation | Guo J, Zhang F, Gao J, et al. Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of Saikosaponin A. J Cell Mol Med. 2020;24(1):174-188. |
Metabolite |
