Study M677

Study name

Yu M 2020b

Title

[The regulatory effects of Chaihu-Shu-Gan-San on fecal metabolome and gut microbiota in depression model rats]

Overall design

The aim of this study was to investigate the antidepressant effects of Chaihu-Shu-Gan-San against chronic variable stress (CVS)-induced depression rats using ultra-performance liquid chromatography coupled with time of flight mass spectrometry (UPLC-Q-TOF/MS) based metabolome approaches. Wistar rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) CVS group, (3) CVS + Chaihu-Shu-Gan-San group (stressor plus Chaihu-Shu-Gan-San water extracts at the dose of 7.0 g/kg), and (4) CVS + fluoxetine group (stressor plus fluoxetine at the dose of 3 mg/kg). The CVS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Fecal samples were used for metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Wistar rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

32

Tissue

Peripheral; Faece; Faece;

Platform

MS-based; LC-MS: not reported;

PMID

N/A

DOI

10.13220/j.cnki.jipr.2020.03.009

Citation

Yu M, Jia H, Zhang H, et al. [The regulatory effects of Chaihu-Shu-Gan-San on fecal metabolome and gut microbiota in depression model rats]. J Int Pharm Res 2020;47(3):229-235. (Article in Chinese)

Metabolite

Chenodeoxycholic acid;

Hypoxanthine;

Nicotinic acid;

5-Methoxytryptophan;

Deoxycholic acid;

Dextrorphan O-glucuronide;

Urobilin;

PS(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z));

L-Urobilinogen;

MG(0:0/20:3(5Z,8Z,11Z)/0:0);

MG(0:0/20:4(8Z,11Z,14Z,17Z)/0:0);

Stearyl citrate;