Study name | Tao X 2020 |
Title | Homeostasis imbalance of microglia and astrocytes leads to alteration in the metabolites of the kynurenine pathway in LPS-induced depressive-like mice |
Overall design | The aim of this study was to evaluate the effect of different doses of lipopolysaccharide (LPS) on depressive-like behaviors, neuroplasticity, the immune system, and kynurenine pathway in the brain of mice. C57BL/6J mice were divided into the following 2 groups (n = 12 in each group): (1) control group (vehicle), and (2) LPS group (single injection of LPS, 0.8 mg/kg, i.p.). The levels of the two metabolites of the kynurenine pathway, 3-hydroxykynurenine and kynurenic acid, in the brain (cerebral cortex and hippocampus) were analyzed by LC-MS/MS (n = 7 per group). |
Type1; | |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model; |
Criteria for depression | Tail suspension test |
Sample size | 14 |
Tissue | Central; Brain; Cerebral cortex; Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: SHIMADZU Prominence LC system (Kyoto, Japan) with QTRAP 5500 mass spectrometer (AB SCIEX, Foster City, CA, USA); |
PMID | |
DOI | |
Citation | Tao X, Yan M, Wang L, et al. Homeostasis imbalance of microglia and astrocytes leads to alteration in the metabolites of the kynurenine pathway in LPS-induced depressive-like mice. Int J Mol Sci. 2020;21(4):1460. |
Metabolite |