Study M659

Study name

Tao X 2020

Title

Homeostasis imbalance of microglia and astrocytes leads to alteration in the metabolites of the kynurenine pathway in LPS-induced depressive-like mice

Overall design

The aim of this study was to evaluate the effect of different doses of lipopolysaccharide (LPS) on depressive-like behaviors, neuroplasticity, the immune system, and kynurenine pathway in the brain of mice. C57BL/6J mice were divided into the following 2 groups (n = 12 in each group): (1) control group (vehicle), and (2) LPS group (single injection of LPS, 0.8 mg/kg, i.p.). The levels of the two metabolites of the kynurenine pathway, 3-hydroxykynurenine and kynurenic acid, in the brain (cerebral cortex and hippocampus) were analyzed by LC-MS/MS (n = 7 per group).

Study Type

Type1;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model;

Criteria for depression

Tail suspension test

Sample size

14

Tissue

Central; Brain; Cerebral cortex;

Central; Brain; Hippocampus;

Platform

MS-based; LC-MS: SHIMADZU Prominence LC system (Kyoto, Japan) with QTRAP 5500 mass spectrometer (AB SCIEX, Foster City, CA, USA);

PMID

32098023

DOI

10.3390/ijms21041460

Citation

Tao X, Yan M, Wang L, et al. Homeostasis imbalance of microglia and astrocytes leads to alteration in the metabolites of the kynurenine pathway in LPS-induced depressive-like mice. Int J Mol Sci. 2020;21(4):1460.

Metabolite

Hydroxykynurenine;