Study M580

Study name

Zhang W 2019

Title

Changes in neurochemical metabolism measured by magnetic resonance spectroscopy and expression of the ERK signaling pathway in a rat model of depression

Overall design

The aim of this study was to explore the neurochemical metabolism in the brain of rats with chronic unpredictable mild stress (CUMS) using magnetic resonance spectroscopy (MRS). Sprague-Dawley rats were divided into the following 2 groups: (1) control group (n = 10), and (2) CUMS group (n = 16). The CUMS stress procedure lasted for 6 weeks. The signals of N-acetylaspartate, choline-containing compounds, glutamate, and myo-inositol were determined by PRESS sequence acquisition 1H spectra in the hippocampus and prefrontal cortex. Metabolite quantification was reported relative to total creatine as the reference peak.

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

26

Tissue

Central; Brain; Prefrontal cortex;

Central; Brain; Hippocampus;

Platform

MRS; MRS: 7.0 T animal MRI scanner (70/20 PharmaScan, Bruker Biospin GmbH, city, Germany);

PMID

N/A

DOI

N/A

Citation

Zhang W, Yang C, Li X, et al. Changes in neurochemical metabolism measured by magnetic resonance spectroscopy and expression of the ERK signaling pathway in a rat model of depression. Int J Clin Exp Med. 2019;12(12):13592-13600.

Metabolite

N-Acetyl aspartate/(Creatine and Phosphocreatine) ratio;

Choline-containing compounds/(Creatine and Phosphocreatine) ratio;

Glutamate/(Creatine and Phosphocreatine) ratio;