Study M576
Study name | Yu DF 2019 |
Title | Chronic unexpected mild stress destroys synaptic plasticity of neurons through a glutamate transporter, GLT-1, of astrocytes in the ischemic stroke rat |
Overall design | The aim of this study was to investigate whether chronic unpredictable mild stress (CUMS) inhibits synaptic formation of regenerated neurons through a glutamate transporter, GLT-1, of astrocytes in the ischemic stroke rats. Middle cerebral artery occlusion (MCAO) model and CUMS model were established as a post-stroke depression model. Sprague-Dawley rats were divided into the following 4 groups: (1) control group, (2) MCAO group, (3) MCAO + CUMS group, and (4) MCAO + CUMS + Ceftriaxone group. The CUMS stress procedure lasted for 18 days after artery occlusion operation, and 200 mg/kg Ceftriaxone was administrated in 1.0 ml 0.9% saline (intraperitoneal injection, i.p.) once a day for 7 days. Cerebrospinal fluid glutamate and glutamine levels were determined by mass spectrometry (n = 5 per group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Sucrose preference test |
Sample size | 20 |
Tissue | Central; Cerebrospinal fluid; Cerebrospinal fluid; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Yu D, Cheng Z, Ali AI, et al. Chronic unexpected mild stress destroys synaptic plasticity of neurons through a glutamate transporter, GLT-1, of astrocytes in the ischemic stroke rat. Neural Plast. 2019;2019:1615925. |
Metabolite |
